不同浓度安氟醚对低温下心肌能量代谢的影响  

Effects of different concentrations of enflurane on myocardial energy metabolism at hypothermia or normothermia

作　　者：高鸿[1] 安裕文[1] 陈恪刚[1] 方开云[1] 高巨[1] 

机构地区：[1]贵阳医学院附属医院麻醉科,550004

出　　处：《中华麻醉学杂志》2000年第7期429-431,共3页Chinese Journal of Anesthesiology

摘　　要：目的研究不同浓度安氟醚（Ｅｎｆ）对常温或低温下心肌能量代谢的影响。方法２４只家兔的离体心脏， Ｌａｎｇｅｎｄｏｒｆｆ装置平衡灌注 １５ｍｉｎ后，随机分为 ４组（ ｎ＝ ６）， Ａ组：常温 １． １％ Ｅｎｆ的Ｋ－Ｈ液灌注； Ｂ组：常温 ２． ２％ Ｅｎｆ的 Ｋ－Ｈ液灌注； Ｃ组：低温 １． １％ Ｅｎｆ的 Ｋ－Ｈ液灌注； Ｄ组：低温２． ２％ Ｅｎｆ的 Ｋ－Ｈ液灌注。常温组温度为 ３７℃，低温组温度为 ３０℃。灌注 ３０ｍｉｎ后，取心失部心肌组织测定腺苷酸含量、ＡＴＰ酶活性。结果常温或低温下， ２． ２％ Ｅｎｆ组 ＡＴＰ含量明显高于 １． １％ Ｅｎｆ组，Ｎａ＋－Ｋ＋ＡＴＰａｓｅ活性明显低于１．１％ Ｅｎｆ组（Ｐ＜０．０５或０．０１）；而低温下，２．２％ Ｅｎｆ组ＡＤＰ含量及ＥＣ明显高于１．１％ Ｅｎｆ组，Ｃａ２＋ＡＴＰａｓｅ活性明显低于１．１％ Ｅｎｆ组（Ｐ＜０．０５或０．０１）。结论 Ｅｎｆ通过抑制心肌细胞膜ＡＴＰａｓｅ活性导致心脏电生理受到抑制，低温可能加重安氟醚的心肌抑制作用。Objective To investigate the effects of enflurane (Enf)at normothermia or hypothermia on myocardial energy metabolism. Methods Twenty-four isolated rabbit hearts, perfused with modified Krebs-Hensleit buffer using Langendorff's device in 15 min, were randomly assigned to be perfused with 1. 1 % Enf at normothermia (37℃ ), 2. 2 % Enf at normothermia (37℃ ), 1. 1 % Enf at hypothermia (30℃ ), or 2. 2 % Enf at hypothermia (30 ℃ ), respectively. The myocardial adenine nucleotides contents, activities of ATPases were measured after 30min perfusion. Results In group 2.2% Enf, ATP content was significantly higher and the activity of Na+ -K+ ATPase was significantly lower than those in group 1. 1 % Enf at normothermia or hypothermia (P < 0. 05 or 0. 01 ); but ADP content and EC in group 2. 2 % enflurane were obviously higher and activity of Ca2+ ATPase was lower than those in group 1. 1 % Enf at hypothermia (P < 0. 05 or 0. 01 ). Conclusions The Enf-mediated depressions of cardiac eletrophysiology and contractility may result from inhibiting the activity of cellular membrane ATPase to reduce myocardial energy metabolism, which can be aggravated by hypothermia.

关 键 词：低温 能量代谢 心肌 安氟醚 

分 类 号：R971.2[医药卫生—药品]