中国汉族群体人类补体C8A多态性  

Genetic polymorphism of human complement C8A in the Chinese Han population and a new allele

作　　者：张林[1] 裴黎[2] 李荣华[1] 陈国弟[1] 吴梅筠[1] 

机构地区：[1]华西医科大学法医物证教研室,成都610041 [2]公安部物证鉴定中心,北京100038

出　　处：《中国法医学杂志》2000年第2期72-74,共3页Chinese Journal of Forensic Medicine

摘　　要：采用免疫沉淀、SDS-聚丙烯酰胺凝胶电泳 (SDS- PAGE)、被动转印及酶免分析 ,研究了人类补体 C8A等位基因频率在成都地区汉族群体中的分布。 12 1份样本被分为 3种常见型 ,即 C8A- A、C8A- B及 C8A- AB,由两个等位共显性基因 C8A * A及 C8A* B控制 ;同时发现了 2个稀有亚型 ,即 A3亚型及新发现的 Ax亚型。等位基因频率为 C8A* A=0 .5 0 83,C8A* B=0 .4835 ,C8A*稀有型 =0 ,0 0 83。说明 C8A多态性在中国群体中具有良好的分布 ,个人识别率(DP)达到 6 1.14% 。Genetic polymorphism of human C8A has been investigated by immunoprecipitation,and separation on sodium dodecylsulfate polyacrylasmide gel electrophoresis (SDS-PAGE)under non-reducing conditions, followed by west-blotting with immunoassay.In 121 samples from unrelated individuals of Chinese Han population,three common phenotypes and two rare phenotypes controlled by two common alleles, C8A*A and C8A*B,and two rare alleles which were designated C8A*A3 and C8A*Ax for acidic variants were observed.The C8A*Ax is a new rare allele.The gene frequencies were:C8A*A 0.5083,C8A*B 0.4835,C8A*A3 and Ax 0.0083,respectively.

关 键 词：补体C8A 多态性 汉族群体 个人识别亲子鉴定 

分 类 号：D919[医药卫生—法医学]