LC-MS/MS法分析北五味子中的木脂素成分  

Composition Analysis of Lignans from Schisandra Chinensis(Turcz) Baill by LC-MS/MS

作　　者：田宏哲[1] 徐静[2] 胡睿[1] 赵瑛博[1] 刘文娥[1] 

机构地区：[1]沈阳农业大学分析测试中心,沈阳110866 [2]辽宁出入境检验检疫局,大连116001

出　　处：《天然产物研究与开发》2012年第B12期32-35,40,共5页Natural Product Research and Development

基　　金：辽宁省博士启动基金(20101100)

摘　　要：建立了同时测定北五味子果实中6种木脂素含量的高效液相色谱-离子阱质谱方法。样品经正己烷提取,提取液过滤后通过旋转蒸发仪浓缩近干,用甲醇溶解,然后采用LC-MS/MS进行测定。6种木脂素在0.005～10.0 mg/g范围内呈良好线性,相关系数为0.9907～0.9998。6种木脂素的定量限为4.0×10-4～1.5×10-3mg/g,平均添加回收率为81.01%～92.45%,相对标准偏差为3.05%～7.98%。采用本文所建立的方法对北五味子果实进行测定,获得了很好的分离效果,分析结果令人满意。A specific liquid chromatography-ion trap mass spectrometric（LC-MS/MS） method was developed that allowed simultaneous determination of 6 lignans in the fruits of Schisandra chinensis（Turcz） Baill.The powder of Schisandra chinensis（Turcz） Baill fruit was extracted with hexane by ultrasonic.The extract was evaporated to almost dryness by a rotatory evaporator after filtration,redissolved by methanol,and then determined by LC-MS/MS.The method showed high degree of linearity（correlation coefficients were 0.9907-0.9998） over the range 0.005-10.0 mg/g for the targets.The limit of quantifications for the lignans ranged from 4.0×10-4 to 1.5×10-3 mg/g.The average recoveries of the lignans in spiked samples were between 81.01%-92.45% with relative standard deviations from 3.05% to 7.98%.The developed method was applied to detect the targets in the Schisandra chinensis（Turcz） Baill fruit with good separation efficiency,and the results of the determination were satisfactory for the samples.

关 键 词：北五味子 木脂素 液相色谱-串联质谱 

分 类 号：O654.7[理学—分析化学]