低磷和铝毒胁迫对大豆谷胱甘肽代谢的影响  

Effects of Low Phosphorus Availability and Aluminium Toxicity on Homoglutathione Metabolism of Soybean

作　　者：李艳英[1,2] 董登峰[1] 王沿沿[1] 李猷[1] 冯慧[1] 

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西农业科学院经济作物研究所,广西南宁530007

出　　处：《西南农业学报》2012年第6期2026-2032,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30960209;31160054)

摘　　要：以磷高效和耐铝毒的大豆双抗品种巴西10号(简称BX10)及双感品种本地2号(简称BD2)为材料,研究低磷和铝毒胁迫下,大豆根部谷胱甘肽(hGSH)含量和氧化还原状态(hGSH/hGSSG)及谷胱甘肽转移酶(GST)、谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GPX)活性的变化规律,并对BX10中谷胱甘肽转移酶(GST)、谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GPX)基因进行半定量表达分析。结果表明:低磷和铝毒胁迫均提高了大豆hGSH含量和hGSH/hGSSG比率及GST、GR和GPX的活性,且BX10上升幅度均要大于BD2;低磷和铝毒胁迫过程均涉及到GST、GR、GPX基因的差异表达,GST基因主要响应铝毒胁迫,GPX基因主要响应低磷胁迫。表明谷胱甘肽代谢与大豆磷高效和耐铝毒能力密切相关。In this study, the soybean variety Brazil No. 10 （BX10）, which exhibit stronger resistance to both phosphorus deficiency and aluminum toxicity, and the contrasting soybean variety BD2 were cultivated in 1/5 Hoagland and used to investigate the changes in the reduced homoglutathione （hGSH） content, oxidative/reductive status, and activities of glutathione S-tranferase（ GST）, glutathione reductase （ GR）, and glutathiane peroxidase （GPX） under low phosphorus availability and aluminum toxicity. The result showed ： （i） both low phosphorus availability and aluminum toxicity increased the hGSH content, oxidative/reductive ratio, and the activities of GST, GR, and GPX; BX10 showed a higher increase percentage than BIY2 in comparison to the corresponding control groups; （ii） gene expression patterns of GST, GR, and GPX involved in glutathione metabolism in response to low phosphorus availability and aluminum toxicity were also determined and analyzed, in which GST displayed the higher expression quantity under aluminum toxicity in roots and GPX exhibited the higher expression quantity under aluminum toxicity. This suggested that soybean glutathione metabolism was highly correlated to low phosphorus availabillty and aluminum toxicity.

关 键 词：大豆 缺磷 铝毒 谷胱甘肽代谢 基因表达 

分 类 号：S565.1[农业科学—作物学]