橡胶树胶乳黄色体死皮相关蛋白的鉴定及分析  

Identification and Analysis of Proteins in Latex Lutoid Associated with Tapping Panel Dryness of Hevea brasiliensis

作　　者：周雪梅[1,2] 杨礼富[1] 王真辉[1] 邹智[1] 袁坤[1] 

机构地区：[1]中国热带农业科学院橡胶研究所,农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737 [2]海南大学农学院,海南海口570100

出　　处：《西南农业学报》2012年第6期2093-2097,共5页Southwest China Journal of Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项(YW-FZX2008-12;2009-12;2010-9);海南省重点科技项目(90107);现代农业产业技术体系建设专项资金(CARS-34-02A)

摘　　要：本实验通过双向凝胶电泳技术(2-DE)比较橡胶死皮树与健康树胶乳黄色体蛋白表达谱的差异,通过软件分析比较获得37个差异表达的蛋白点,经基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)分析后,搜索NCBInr数据库,结果表明,有11个蛋白点被成功鉴定,其中7个为未知蛋白,其余4个蛋白分别为chloroplast 23 kDa polypeptide of photosystem II、ATP synthase beta chain、NADP-dependent sorbitol-6-phosphate dehydrogenase和vitamin-b12 independent methionine synthase。这4个蛋白在死皮树中表达量均下调,可能在死皮发生过程中起着重要作用。本研究将为进一步阐明橡胶树死皮发生的分子机制提供理论依据。In this experiment, the two-dimensional gel electrophoresis（2-DE） was used to study the differentiation of protein expression profiles of latex lutoid between TPD trees and healthy ones in Hevea brasiliensis. 37 differentially expressed protein spots were obtained by software analysis. After these spots were subjected to matrix assisted laser deserption/ionization time of flight mass spectrometry （ MALDI-TOF MS） followed by searching for NCBInr database, the result showed that 11 spots were successfully identified, of which 7 were identified as the unknown proteins, and the remaining 4 were the chloroplast 23 kDa polypeptide of photosystem II, ATP synthase beta chain, NADP-dependent sorbitol-6-phosphate dehydrogenase and vitamin-bl2 independent methionine synthase. There were some relationship between osmotin protein and TPD broom according to the functions of down-regulated proteins in TPD trees. This research provided a theoretical basis for TPD.

关 键 词：橡胶树 死皮 双向凝胶电泳 蛋白 质谱 

分 类 号：S794.1[农业科学—林木遗传育种]