甘蔗试管苗瓶外生根技术的研究  

Studies on Ex Vitro Rooting of Sugarcane Microshoots

作　　者：何为中[1] 陈引芝[1] 刘红坚[1] 李松[1] 汪淼[1] 翁梦苓[1] 王天算[1] 苏树权[1] 余坤兴[1] 

机构地区：[1]广西农业科学院甘蔗研究所,广西甘蔗遗传改良重点实验室,中国农业科学院甘蔗研究中心,广西南宁530007

出　　处：《西南农业学报》2012年第6期2117-2120,共4页Southwest China Journal of Agricultural Sciences

基　　金：广西自然科学基金项目(桂科回0991012);广西科学研究与技术开发项目(桂科合1140011-3);广西农业科学院科技发展基金项目(桂农科2012JM04)

摘　　要：为优化甘蔗试管苗瓶外生根的环境条件,提高成活率,降低生产成本,进行了外界环境因素对甘蔗无根试管苗瓶外生根及生长的影响研究。结果表明,高肥力基质(如商品基质)处理的无根试管苗成活率低,但成活植株生长快,植株高;而低肥力基质(如新鲜河沙土)有利于无根试管苗生根成活,但植株生长慢;适合甘蔗无根试管苗移栽的基质包括新鲜河沙土、田园土和河沙与田园土的混合物。温度、光照和湿度是甘蔗试管苗瓶外生根维持高存活率的关键外部因素,利用遮阳网和盖膜等措施调节温度、湿度和光照可使试管苗的生长环境逐渐过渡到自然环境条件。试管苗瓶外生根技术有利于简化传统甘蔗试管苗生产,降低生产成本,促进甘蔗健康种苗的推广应用。The current research aimed at exploring the external factors affecting the ex vitro rooting in micro-shoots, simplifying the rooting method, and reducing the production costs. The non-rooted sugarcane micro-shoots, sub-cultured on GA3 （ 1.5 mg· L^- 1 ） supplemented MS medium for 15 days, were transplanted into greenhouse environment after chemical sterilization. The results showed that the high fertile substrate （commercial） was not beneficial for higher survival rate but promoted the growth of plants, while the lower fertile substrate （sand） was not beneficial for the growth of shoot but resulted in higher survival rate. The temperature, humidity, and light intensity of ex vitro microenvironment should be changed gradually to natural conditions during acclimatization through plastic film covering, shading net, and proper water management after transplanting so that the sugarcane micro-shoot could be well adjusted to natural conditions. In addition, the in vitro rooting shoots showed higher survival rate and better growth than the ex vitro shoots. The current study will assist in the development techniques for attaining higher survival rate of micro-shoots and ultimately reduce the cost of disease-free tissue cultured sugarcane plantlets large scale production.

关 键 词：甘蔗 无根试管苗 瓶外生根 存活率 

分 类 号：S566.1[农业科学—作物学]