双孢蘑菇和蘑菇的ITS-RFLP分析  

Studies on Agaricus bisporus and Agaricus campestris Using ITS-RFLP

作　　者：王波[1] 刘勇[1,2] 赵小青[1,3] 王一[1,2] 王泽生[4] 

机构地区：[1]四川省农业科学院土壤肥料研究所,四川成都610066 [2]四川农业大学资源环境学院,四川雅安625014 [3]四川大学生命科学院,四川成都610041 [4]福建省农业科学院食用菌研究所,福建福州350014

出　　处：《西南农业学报》2012年第6期2222-2226,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30671456和30771503);农业部948项目(2006-G11(A)-01);农业部公益性行业(农业)科研专项(nyhyzx07-008);四川省科技厅食用菌育种攻关(05NG001-016-2)

摘　　要：对采自四川和西藏的双孢蘑菇(Agaricus bisporus)和蘑菇(A.campestris)9个野生菌株,以及双孢蘑菇5个栽培菌株为材料,进行了ITS-RFLP分析。结果表明,ITS-PCR条带约为750 bp,5种限制性内切酶(MspⅠ、HinfⅠ、HaeⅡ、AulⅠ、TaqⅠ)的ITS-RFLP分析结果为,只有4种限制性内切酶(HinfⅠ、HaeⅡ、AulⅠ、TaqⅠ)产生多态性条带,但完全能将双孢蘑菇和蘑菇区分的限制性内切酶为TaqⅠ;对5种限制性内切酶的ITS-RFLP分析数据进行聚类结果为,在相似系数为0.615水平以下分为2个类群,并将双孢蘑菇和蘑菇区分开;在相似系数为0.915水平上,供试的11个野生和栽培双孢蘑菇菌株分为3个亚类群。14 Agaricus strains, including 9 wild strains of Agarlcus bisporus or Agaricus campestris and 4 cultivation strains of Agaricus bisporus, were studied by using ITS-RFLP. The results showed that a band with 750 bp from the strains was respectively amplified by the primer combination of 1TS4 and ITSS, and polymorphic bands were obtained after digestive treatments with restriction enzymes of HinfI , Hae II, Aul I , Taq I , but no polymorphic bands produced with Msp I, and Agaricus bisporus strains could be distinguished from Agarieus campestris strains only using Taq I. The cluster results indicated that strains ofAgaricus bisporus and Agaricus campestris could be distinguished and divided into two groups at similarity coefficient of 0. 615, and the strains ofAgaricus bisporus were divided into three subgroups at similarity co-efficient of 0.915.

关 键 词：野生 双孢蘑菇 蘑菇 ITS RFLP 

分 类 号：S646.1[农业科学—蔬菜学]