类猪圆环病毒因子P1在自然感染仔猪体内的组织分布  

Distribution of porcine circovirus-like agent P1 in a variety of tissues from naturally infected piglets

作　　者：温立斌[1] 何孔旺[1] 俞正玉[1] 茅爱华[1] 高晓静[1] 倪艳秀[1] 张雪寒[1] 郭容利[1] 李彬[1] 王小敏[1] 周俊明[1] 吕立新[1] 

机构地区：[1]江苏省农业科学院兽医研究所农业部动物疫病诊断与免疫重点开放实验室国家兽用生物制品工程技术研究中心,江苏南京210014

出　　处：《中国兽医学报》2013年第1期1-3,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972184);江苏省自然科学基金资助项目(BK2008351)

摘　　要：选用3头自然感染仔猪和1头阴性对照猪,剖杀后采集心脏、肝脏、脾脏、肺脏、肾脏、胰脏、脑、空肠、扁桃体、胸腺、淋巴结、膀胱、性腺等组织,用荧光定量PCR方法检测病毒在组织中的分布及病毒载量。结果显示,类猪圆环病毒因子P1分布在仔猪的心脏、肝脏、肺脏、胰脏、脑和膀胱等组织,病毒载量以胰脏、脑和膀胱为最高,可达105拷贝/g以上;对照猪脏器中没有检测到病毒核酸。该研究为P1的诊断及致病机制奠定了基础。The objectives of this study were to investigate the distribution of porcine circovirus-like agent P1 in the tissues of piglets.Three naturally infected pigs and one negative-control pig were selected and sacrificed.The presence and viral load of P1 in collected tissue samples（including mesenteric and superficial inguinal lymph nodes,thymus,tonsil,heart,lung,kidney,liver,spleen,brain,pancreas,bladder,jejunum and gonad） were determined by real-time quantitative PCR.The results indicate that P1 may be distributed in heart,lung,liver,brain,pancreas and bladder.Across these tissues of naturally infected pigs,the brain,pancreas and bladder had the highest viral burdens with 105copies/g.Results from this study lay the foundation for diagnosis and pathogenesis studies of P1.

关 键 词：类猪圆环病毒因子P1 组织分布 病毒载量 

分 类 号：S858.285.3[农业科学—临床兽医学]