鸭瘟病毒强毒株感染SPF鸭后动态病理组织学变化  

Dynamic change of histopathology of SPF duck infected with duck plague virus virulent strain

作　　者：张坤[1] 刁有祥[1] 程彦丽[1] 崔京腾[1] 王蛟[1] 

机构地区：[1]山东农业大学,动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2013年第1期9-15,48,共8页Chinese Journal of Veterinary Science

基　　金：现代农业产业技术体系资助项目(CARS-43-34)

摘　　要：用鸭瘟病毒(Duck plague virus,DPV)人工感染2月龄SPF鸭,定期剖杀,经聚合酶链反应(PCR)检测为鸭瘟后,对各组织器官的病理组织学变化进行观察,并进行血常规和血液生化指标检测。结果显示,人工感染后24h,试验鸭中枢免疫器官胸腺、法氏囊表现为淋巴细胞数量减少,组织间隙增大;肝脏、脾脏组织病变较为严重,大部分组织器官均出现程度较轻的病理变化。感染后48～96h,中枢免疫器官的淋巴细胞极度减少、网状细胞增生、组织器官结构模糊不清,严重充血、出血;其余组织器官出现细胞变性、出血等不可逆病理变化。感染后120h,组织细胞变性、坏死,出现大片坏死区。点眼滴鼻组鸭感染DPV后组织学变化与皮下注射组相似,只是发生的时间偏后约24～48h。对照组鸭病理组织学观察未见损伤。WBC、HGB、AST、ALT等发生显著变化。结果表明,接种DPV强毒感染鸭的组织器官严重受损,特别是免疫器官,甚至会引起免疫抑制。Two-month-old SPF ducks were inoculated with duck plague virus（DPV） and slaughtered at different period.After determined virulent DPV by PCR,the histopathology changes of tissues and organs were observed.And the blood were collected to be carried out the routine blood test and the detection of blood parameters.The results showed that at 24 h post inoculation,the number of lymphocyte in central immune organs experimental ducks,including thymus and bursa,decreased and tissue space enlarged.The histopathologic damage of liver and spleen was serious,other organs was slight.48-96 h post inoculation,the number of lymphocyte decreased severely in central immune organs,hyperplasia of lymphocytic netted cell,blurry configuration,serious hyperemia and hemorrhage,some irreversible pathological changes,cell degeneration and hemorrhage,were observed.At 120 h post inoculation,cell degeneration and focal necrosis of tissue appeared.Histological changes of ducks infected by conjunctival and intronasal vaccination were similar to those subcutaneous injection,but the onset was delayed about 24-48 hours.Histopathology of control group was not observed.WBC,HGB,AST,ALT in blood of the inoculation ducks showed obvious changes.The result showed tissues and organs,especially the immune organs,of SPF ducks inoculated DPV severely damaged,which even resulted in immunosuppression.

关 键 词：鸭瘟病毒 PCR 病理组织学变化 血常规和血生化检测 

分 类 号：S852.657[农业科学—基础兽医学]