基于线粒体CO2和ND4基因对四川省豆状带绦虫种群遗传多样性的分析  

Population genetic diversity based on CO2 and ND4 genes in Taenia pisiformis from Sichuan

作　　者：杨德英[1] 任永军[2] 聂华明[1] 付彦[1] 谢跃[1] 陈林[1] 农向[1] 岩宁[1] 古小彬[1] 王淑贤[1] 杨光友[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]四川省畜牧科学研究院,四川成都610066

出　　处：《中国兽医学报》2013年第1期43-48,共6页Chinese Journal of Veterinary Science

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(TRT0848)

摘　　要：利用PCR扩增了中国四川省7个不同地理区域(雅安、攀枝花、乐山、广元、泸州、广安和阿坝)23株家兔体内豆状囊尾蚴的CO2(Cytochrome oxidase subunit 2,468bp)和ND4(NADH dehydrogenase subunit 4,1 077bp)基因的部分序列,并进行分析。结果显示,7个种群23个分离株中CO2和ND4基因序列中分别有21个和32个变异位点,分别检测出了5个和7个单倍型,2个基因的种群分析结果显示乐山种群最为保守;CO2分析结果显示攀枝花和雅安种群内变异最大,且高于种群间变异。2个线粒体基因构建的NJ/MP树显示7个种群的系统发生与他们分布的地理区域没有直接的相关性,还未形成显著的地理种群结构。结果表明,四川省7个地理种群间和种群内豆状囊尾蚴均存在一定的遗传变异和遗传多样性,为豆状带绦虫的分子流行病学调查和群体遗传研究奠定了基础。The objectives of the present paper were to determine the level of genetic variation and population genetic diversity of rabbit Cysticercus pisiformis from seven different regions（Ya＇an,Panzhihua,Leshan,Guangyuan,Luzhou,Guang＇an,and Aba） in Sichuan,China.Partial sequences of the mitochondrial CO2（468 bp） and ND4（1 077 bp） gene of 23 isolates of C.pisiformis were obtained by PCR and analyzed.The results showed that there were 21 variable sites and five haplotypes in CO2 sequences,32 variable sites and seven haplotypes in ND4 sequences.Leshan population was the most conserved based on analyses of population diversity using CO2 and ND4 genes.According to CO2 gene,the maximum intrapopulation genetic diversity occurred in Panzhihua and Ya＇an population,and was higher than intraspecific genetic diversity.NJ/MP trees suggested that there was no direct correlation between phylogeny of seven populations and geographical regions.The results indicate that the certain genetic variation and heredity diversity were presented in seven geographical populations among intrapopulation and intraspecific C.pisiformis,and provide foundation for research on epidemiological survey and population genetic diversity.

关 键 词：豆状囊尾蚴 CO2 ND4 种群遗传多样性 中国四川 

分 类 号：S852.734[农业科学—基础兽医学]