2种方法诱导形成的破骨细胞特性比较  

Characteristics comparison of osteoclasts induced by M-CSF and RANKL

作　　者：付应霄[1] 顾建红[1] 王世涛[1] 王怡[1] 赵鸿雁[1] 刘伟[1] 仝锡帅[1] 刘宗平[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2013年第1期94-97,101,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972229;31172373);江苏省高校优势学科建设工程资助项目;教育部博士点基金资助项目(20113250110003)

摘　　要：采集ICR小鼠骨髓单核细胞在体外培养过程中加入25.0μg/L M-CSF+50.0μg/L RANKL诱导形成破骨细胞,同时选择RAW264.7细胞培养过程中添加100.0μg/L RANKL诱导分化为破骨细胞。通过检测抗酒石酸碱性磷酸酶(Tartrate resistant acid phosphatase,TRAP)染色阳性数及骨吸收陷窝,比较小鼠骨髓单核细胞和RAW264.7细胞株诱导形成的破骨细胞活性。结果显示,小鼠骨髓诱导的细胞TRAP阳性数显著低于RAW264.7细胞株(P<0.05),而小鼠骨髓诱导的破骨细胞所形成骨吸收陷窝的面积显著大于RAW264.7细胞株(P<0.05)。结果表明,2种方法均可诱导形成具有典型特征的破骨细胞,骨髓单核细胞诱导形成的破骨细胞活性更强,但破骨细胞的数量和纯度明显低于RAW264.7细胞株。The biological characteristics of osteoclasts were induced from mouse bone marrow monocytes and RAW264.7 cells were compared by tartrate resistant acid phosphatase（TRAP） staining and bone resorption pits assaying in this paper.Collecting ICR mouse bone marrow monocytes,osteoclasts were formed by inducation bone marrow monocytes with 25.0 μg/L M-CSF＋50.0 μg/L RANKL in vitro,at the same time,osteoclasts were formed by inducation RAW264.7 cells with 100.0 μg/L RANKL.The results showed TRAP positive multinuclear cells induced from bone marrow monocytes were less than from RAW264.7 cells significantly（P0.05）,conversely,the area of bone resorption pits was different significantly（P0.05）.The cells induced by two methods displayed typical characteristics of osteoclasts,their activity of TRAP was stronger in the cells induced by bone marrow monocytes,but the number and purity of cells induced by RAW264.7 cells were higher.

关 键 词：小鼠骨髓单核细胞 RAW264 7细胞株 破骨细胞 特性 

分 类 号：S852.1[农业科学—基础兽医学]