微卫星DNA标记技术对试验用虎皮猫群体遗传结构的分析  

Analyzsis of population genetic structure of laboratory orange tabby cat by microsatellite DNA markers

作　　者：衣帅[1] 谢姗珊[1] 刘继峰[2] 杜小燕[3] 齐飞虎 李益琛[3] 任文陟[1] 刘殿峰[1] 陈振文[3] 

机构地区：[1]吉林大学实验动物中心,吉林长春130062 [2]华北制药集团新药研究开发有限责任公司,河北石家庄050015 [3]首都医科大学基础医学院,北京100069 [4]华北制药厂股份有限公司,河北石家庄050015

出　　处：《中国兽医学报》2013年第1期137-141,共5页Chinese Journal of Veterinary Science

基　　金：国家"十二五"科技支撑计划资助项目(2011BAI15B01-32);国家自然科学基金资助项目(31172168)

摘　　要：用筛选优化出的24个具有丰富多态性的微卫星位点对华北制药厂的34只虎皮猫基因组DNA进行PCR扩增,对扩增产物进行琼脂糖凝胶电泳和STR扫描,运用popgene3.2软件对试验用虎皮猫群体进行群体遗传结构分析。结果显示,虎皮猫群体平均观测等位基因数为4.083 3,平均有效等位基因数为2.632 3,平均有效杂合度为0.610 8,平均香隆指数为1.078 6。结果表明,微卫星DNA标记技术适于猫群体遗传结构分析;该试验用虎皮猫群体符合封闭群的遗传特性。To evaluate the genetic structure of the laboratory orange tabby cat population.Twenty four refined polymorphic microsatellite loci were applied to amplify the genomic DNA from 34 orange tabby cats in Huabei Pharmaceutical factory by PCR.The PCR products were analyze by agarose gel electrophoresis,and short tandem repeat（STR） scanning.Software popgene 3.2 was used to analyze the genetic structure.For the orange tabby cat population,the observed average number of alleles is 4.083 3;the average effective number of alleles is 2.632 3;the mean effective heterozygosity is 0.610 8;the mean Shannon＇s information index is 1.078 6.The microsatellite DNA markers are suitable for analyzing the genetic structure of cats and the laboratory orange tabby cat population has the characters of closed colony.

关 键 词：微卫星DNA 多态性 虎皮猫 PCR 遗传检测 

分 类 号：S813[农业科学—畜牧学]