鲍曼不动杆菌对β-内酰胺类药物耐药机制的研究  

Study on mechanism of resistance of Acinetobacter bauannii to β-Lactams

作　　者：许静[1] 普珍[2] 孙增先[1] 茆海丰[1] 高山[1] 

机构地区：[1]连云港市第一人民医院临床药学研究中心,江苏连云港222002 [2]西藏大学医学院药学系,西藏拉萨850002

出　　处：《临床和实验医学杂志》2013年第1期20-21,23,共3页Journal of Clinical and Experimental Medicine

基　　金：连云港市卫生局课题;课题编号09051

摘　　要：目的调查耐药鲍曼不动杆菌β-内酰胺酶基因型流行情况,为医院合理使用抗菌药物提供参考依据。方法用聚合酶链反应(PCR)方法对7株鲍曼不动杆菌的8种相关β-内酰胺酶基因型(TEM、AmpC、VIM、IMP、PER、GES、VEB、SHV)进行检测,并通过琼脂凝胶电泳后进行成像验证。结果 7株鲍曼不动杆菌中blaTEM染色体检测阳性有7株、质粒阳性7株;blaAmpC染色体阳性3株、质粒阳性6株;blaPER染色体阳性4株;其余β-内酰胺酶基因未检出,其中有1株同时携带有3种耐药基因型。结论β-内酰胺类药物耐药基因以TEM型最为常见,其次为ampC型、PER型,其余型未见发现;同一株耐药菌可携带多种耐药基因。Objective To study the prevalence of genotypes of muhidrug resistance of Acinetobacter baumannii in this hospital and to pro- vide reference to rational use of antibacterials. Methods Among 7 strains of A. baumannii, 8 kinds of β laetam - resistant genotypes （ TEM, AmpC, VIM, IMP, PER, GES, VEB and SHV） were detected by polymerase chain reaction （PCR） assay, and they were verified by agarose gel electrophoresis. Results In these 7 strains, blaTEM was positive in chromosome and plasmid of 7 strains, blaAmpC was positive in chromosome of 3 strains and plasmid of 6 strains, blaPER was positive in chromosome of 4 strains, and others were negative. There was one strain carrying three genotypes. Conclusion In this hospital, blaTEM was the most important resistant genotype, and blaAmpC, and blaPER were the next, and no other types had been found. A β lactam - resistant strain can carry more than one resistant genotype.

关 键 词：鲍曼不动杆菌 Β-内酰胺类药物 耐药机制 聚合酶链反应 

分 类 号：R446.5[医药卫生—诊断学]