犬骨骼肌卫星细胞的分离、培养及扩增方法探索  

The Isolation, Culture and Multiplication of Skeletal Muscle Satellite Cells

作　　者：张臻[1] 朱洪生[1] 钟竑[1] 卫洪超[1] 

机构地区：[1]上海第二医科大学附属仁济医院胸外科,上海200001

出　　处：《上海实验动物科学》2000年第2期72-75,共4页Shanghai Laboratory Animal Science

基　　金：国家自然科学基金资助项目!(39770735)

摘　　要：为骨骼肌卫星细胞自体移植治疗心肌疾病准备细胞材料 ;建立了体外分离、纯化、培养、扩增骨骼肌卫星细胞的方法。用机械法结合酶消化法分离犬骨骼肌卫星细胞 ,经Percoll非连续密度梯度离心纯化过程后 ,以 Desmin链霉素抗生物素 -过氧化酶免疫细胞化学染色鉴定。以完全培养液加用扩增卫星细胞。提取的卫星细胞纯度在 90 %以上 ,且证明促肝细胞生长素能增强细胞的增殖能力。The authors had isolated skeletal muscle satellite cells by mechanic and enzymic digestion, purified them by Pecoll discontinuous density gradient centrifugation and identified them immunocytochemically by Desmin labeling,and multiplied the satellite cells by administration of promoting heptocyte growth factor (pHGF). The purity of harvested satellite cells exceeded 90% .It was suggested that the pHGF was usful for prompting satellite cell growth by shortening the lag period and enhancing their proliferation capacities.

关 键 词：骨骼肌卫星细胞 分离 培养 扩增 移植 心肌疾病 

分 类 号：Q952[生物学—动物学]