Surgetake^(TM)间皮组织细胞极性修复与癌细胞抑制实验研究  

Surgitake^(TM) for repair of mesothelium polarity and cancer cell inhibition:an experimental study

作　　者：王民 郑青山[2] 王宇[3] 张忠涛[3] 李建设[3] 薛建国[3] 周延忠[3] 赵莉莉[4] 杨喜花[4] 杨永明[4] 阎磊[4] 白喜花[4] 任连生[4] 

机构地区：[1]山西皮尔复临床医药研究所中心研究室,太原030001 [2]上海中医药大学临床药理研究中心 [3]首都医科大学附属北京友谊医院肝胆外科 [4]山西省肿瘤医院肿瘤研究室

出　　处：《中国药物与临床》2013年第1期5-8,I0001,共5页Chinese Remedies & Clinics

基　　金：国家九五攻关项目"国家十项新药与产业化"课题子项目(9690105159)

摘　　要：目的探讨间皮组织细胞极性修复对癌细胞生长抑制的影响。方法分别采用细胞极性修复实验、消化道吻合口愈合影响实验、"T"管窦道形成实验、细胞极性修复相关指标检测、永生化细胞(人膀胱癌细胞株T24)抑制实验等一系列从动物实验到细胞实验,采用SurgetakeTM或生理盐水进行了对照研究。结果兔实验结果显示,实验组10例均实现极性修复,而对照组均无完全实现极性修改;同时实验表明SurgetakeTM具有较强的激活体内t-PA的活性,用药后实验组t-PA达到(1.6±0.8)IU/ml,而对照组t-PA为(0.9±0.4)IU/ml,两组比较差异有统计学意义(P<0.01)。永生化细胞(人膀胱癌细胞株T24)抑制实验实验结果显示surgetakeTM组3个视野计数细胞总数为844个,对照组3个视野细胞总数为2295个。结论 SurgetakeTM具有保持间皮组织极性的作用,实现了间皮结构的重建,能使t-PA增加,细胞极性增强,组织修复的就好。细胞的极性保持过程与小分子蛋白t-PA相关,这一过程的极化作用可使肿瘤细胞恶性程度降低。Objective To determine the effects of repair of mesothelium polarity on cancer cell inhibition.Methods Serial animal and cytological experiments,including cell polarity repair test,alimentary tract anastamosis healing test,T-tube fistula formation test,assessment of the parameters associated with cellular polarity repair and immortal cell（human bladder cancer cell line T24） inhibition,were employed in the control trials by using Surgetake^TM and normal saline.Results The experiments on rabbits evidenced a markedly higher proportion of complete repair of cellular polarity in SurgetakeTM group（n=10） than that in control group（P〈0.01）.Surgetake^TM more potently potentiated the t-PA activity in vivo [（1.6±0.8）IU/ml vs（0.9±0.4）IU/ml],P〈0.01） and yielded a higher cell count in three visual fields when compared with normal controls（2995 vs 844,P〈0.05）,as evidenced by immortalized cell inhibition test.Conclusion Surgetake^TM achieves mesothelium reconstruction by retaining tissue polarity and benefits the tissue repair from elevated t-PA activity leading to potentiated cellular polarity.The fact that maintenance of polarity correlate considerably with t-PA,a low molecular weight protein,indicates reduced malignancy via cellular polarization.

关 键 词：上皮 肿瘤 组织 

分 类 号：R692[医药卫生—泌尿科学]