小球藻病毒基因调控序列在大肠杆菌和真核藻中的调控活性研究  

The Regulation Activity of Chlorella Virus Gene 5' Upstream Sequence in Escherichia coli and Eucaryotic Alage

作　　者：康明[1] 韩继刚[1] 刘平芳[1] 叶寅[1] 田波[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《生物工程学报》2000年第4期443-446,共4页Chinese Journal of Biotechnology

基　　金：海洋"8 63"项目!( 819 0 4 0 9 98);国家自然科学基金项目!( 3 9870 0 16)

摘　　要：以小球藻病毒腺嘌呤甲基转移酶基因 (amt)和主要外壳蛋白VP5 4基因的 5′上游调控序列构建大肠杆菌和真核藻转化载体。以PRPL 及CaMV35S启动子为阳性对照 ,研究了小球藻病毒来源的两种调控序列在E .coli和真核藻细胞中的启动活性。发现PAMT在 4种E .coli菌株中都具有极强的调控活性 ,启动Luc基因表达而产生的酶活性高于PRPL5 0～ 40 0倍 ;PVP54 在DH5α中也具有较强的启动活性。同时PAMT在两种小球藻中启动GUS基因瞬时表达的能力也明显高于CaMV35S启动子 ,表明它们有可能在真核藻类遗传转化中具有很好的应用前景。The 5′ upstream regions of adenine methyltransgerase gene and major coat protein gene (P AMT ,P VP54 ) in Chlorella virus genomes were used to contract transformation vectors in E.coli and eukaryotic algae.The regulation acti vities of P AMT and P VP54 comparing with P RP L and CaMV35S promoters were analyzed in different E.coli strains and Chlorella species.It is found that the luciferase activity controlled by P AMT is 50～400 times higher than that controlled by P RP L.The regulation activity of P AMT in 2 Chlorella species is obviously higher than that of CaMV35S promoter.It is the first report that the 5′ upstream region of Chlorella virus gene has strong regulation activity in eucaryotic algae.The result suggests this regulation sequence will have an excellent application in the eucaryotic algae genetic engineering.

关 键 词：病毒基因启动子 调控活性 真核藻 瞬时表达 

分 类 号：Q786[生物学—分子生物学]