水杨酸在紫杉醇生物合成中诱导作用的研究  

Study on the Effects of Salicylic Acid on Taxol Biosynthesis

作　　者：苗志奇[1] 未作君[1] 元英进[1] 

机构地区：[1]天津大学生化工程系,天津300072

出　　处：《生物工程学报》2000年第4期509-513,共5页Chinese Journal of Biotechnology

基　　金：教育部"跨世纪优秀人才培养计划"基金;天津市科委( 963 10 72 11)项目资助

摘　　要：研究了水杨酸对红豆杉细胞培养中紫杉烷合成的影响。在适宜浓度的水杨酸诱导下 ,紫杉醇 (Taxol)的产量提高了近 3倍 ,同时 10 去乙酰基巴卡亭Ⅲ (10 DAB)与巴卡亭Ⅲ (BaccatinⅢ )相应上升。通过对紫杉醇合成代谢途径的动力学分析 ,初步推断水杨酸的加入提高了 10 DAB合成速率。并通过水杨酸和硝酸银的配伍诱导 ,实现了诱导子之间的协同作用 ,获得了 39mg/L的紫杉醇含量 ,比两个诱导子单独作用时的最高含量之和还高出 5 0 %。The influence of salicylic acid on the production of taxanes in plant cell culture was studied.Experimental results showed that addition of salicylic acid at concentration of 0.1 mg/L could enhance the production of taxol to three fold.The concentration of 10 DAB and baccatin Ⅲ was also increasing while taxol concentration increases under salicylic acid elicitation.On the basis of the kinetic analysis about the simplified taxol biosynthesis pathway,a probable reason that salicylic acid improves the rate of 10 DAB producing reaction was introduced.The results above can direct its inducing mechanic research and provide the basis of multiple elicitors synergism.The concentration of taxol arrives at 39 mg·L -1 induced by salicylic acid with silver nitrate,being 150 percent of the sum of taxol obtained under elicitation of salicylic acid and silver nitrate respectively.

关 键 词：诱导作用 紫杉醇 水杨酸 作用位点 植物细胞培养 

分 类 号：Q251[生物学—细胞生物学]