大肠杆菌耦合乙酸分离的过滤培养  

Cultivation Integrated with Acetate Acid Filtration on Escherichia coli

作　　者：杜鹏[1] 叶勤[1] 俞俊棠[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《生物工程学报》2000年第4期528-530,共3页Chinese Journal of Biotechnology

基　　金：国家自然科学基金重点资助项目!( 2 92 3 614 0 )

摘　　要：A fed batch fermentation integrated with filtration process was proceeded in a synthetic medium with the use of a hollow fibre membrane filter.The activity of α A interferon reached 1.4×10 9u/L,which was increased 320% over that of a control process.The integrated process was then proceeded in the synthetic medium supplemented with yeast extract during the earlier stage.The addition of yeast extract not only reduced the accumulation of acetate,but also promoted the production of α A interferon.The maximum activity achieved 1.9×10 9 u/L during the fermentation,which was increased 480% over that of a normal process.A fed batch fermentation integrated with filtration process was proceeded in a synthetic medium with the use of a hollow fibre membrane filter.The activity of α A interferon reached 1.4×10 9u/L,which was increased 320% over that of a control process.The integrated process was then proceeded in the synthetic medium supplemented with yeast extract during the earlier stage.The addition of yeast extract not only reduced the accumulation of acetate,but also promoted the production of α A interferon.The maximum activity achieved 1.9×10 9 u/L during the fermentation,which was increased 480% over that of a normal process.

关 键 词：发酵分离耦合 乙酸 干扰素 大肠杆菌 E.COLI 

分 类 号：Q93-3[生物学—微生物学]