Biolog细菌自动鉴定系统应用初探  

PRELIMINARY EXPLORATION OF APPLICATION OF BIOLOG AUTOMATED BACTERIAL IDENTIFICATION SYSTEM

作　　者：冯瑞华[1] 樊蕙[1] 李力[1] 葛诚[1] 

机构地区：[1]中国农业科学院土壤肥料研究所,北京100081

出　　处：《微生物学杂志》2000年第2期36-38,共3页Journal of Microbiology

摘　　要：利用BiloogMicrostation细菌自动鉴定系统（3.50版）对已知的9个属23株菌进行了鉴定。24hBiolog系统鉴定结果：12林革兰氏阴性菌中，9株可准确鉴定到种的水平（75％），3株未达到属的水平。11株革兰氏阳性菌均属于芽孢杆菌属，全部鉴定到属的水平，9株准确鉴定到种的水平（81.8％）。总计属的水平准确率86.9％（20／23），种的水平准确率78.2％（18／23）。Twenty-three known isolated bacteria of nine genera were identified by Biolog Mindation Identificatdri System. Nine (75% ) of 12 Gram-negative isolates were corrcetly identifial both at genus and species level in 24 hours. of the 11 Gram-positive isolates of spore-forming rods, were all comeily identified at genus levelt, only 9 (81. 8% ) at species level in 24 hours. Problems encountered in the application were discussed.

关 键 词：Biolog系统 细菌突变 自动鉴定系统 土壤细菌 

分 类 号：S154.381[农业科学—土壤学] S126[农业科学—农业基础科学]