L-半胱氨酸对连续流发酵生物制氢的促进作用  

Enhancement of L-cysteine on continuous fermentative biohydrogen production

作　　者：曲媛媛[1] 任南琪[1] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江哈尔滨150090

出　　处：《哈尔滨工程大学学报》2012年第12期1559-1563,共5页Journal of Harbin Engineering University

基　　金：国家自然科学基金资助项目(51178140);国家创新研究群体基金资助项目(51121062)

摘　　要：为了研究L-半胱氨酸对连续流厌氧发酵生物制氢系统的影响,通过运行两组平行的连续流搅拌槽式反应器(CSTR),一组随进水连续添加0.1 g/L的L-半胱氨酸,另一组不添加,对比考察两组系统的氧化还原电位、生物量、产酸发酵情况以及产氢能力.结果发现添加L-半胱氨酸反应器的氧化还原电位降至-400 mV以下仅需1 d,形成稳定的乙醇型发酵仅需25 d;而未添加L-半胱氨酸的反应器则分别需要10 d和35 d.达到稳定运行状态时,添加L-半胱氨酸反应器的产氢速率为3.06 L/d,高于未添加L-半胱氨酸的2.99 L/d.研究表明L-半胱氨酸能够促进形成适宜乙醇型产氢发酵的低氧化还原电位环境,从而加快连续流产氢系统的启动进程,并提高系统的产氢能力.The research study plans to investigate the influence of L-cysteine on the continuous anaerobic fermentative biohydrogen production system,utilizing two continuously stirred tank reactors（CSTR） operated under two parallel controlling parameters;one added with L-cysteine continuously at 0.1g/L with the inflow of water and the other without any addition.The oxidation-reduction potential（ORP）,biomass,fermentation liquid end metabolites and characteristics of hydrogen production were inspected contrastively.In the reactor with L-cysteine addition,the time taken for ORP to decrease below-400 mV and ethanol-type fermentation formation took 1 day and 25 days respectively,however it took 10 days and 35 days in the other reactor in contrast.In the stable operating stage,the hydrogen production rate was 3.06 L/d in the reactor with L-cysteine addition,higher than 2.99 L/d in the other without addition.The results show that,L-cysteine accelerates the formation of low ORP environment,which is favored by ethanol-type fermentation,because it leads to a quick startup of continuous hydrogen production system,and enhances hydrogen production of the system.

关 键 词：L-半胱氨酸 生物制氢 连续流发酵制氢 厌氧 连续流搅拌槽式反应器 

分 类 号：X703.1[环境科学与工程—环境工程]