马氏钳蝎毒素中多肽BmK4112的分离纯化和一级结构测定  

Purification and Primary Structure of a Novel Peptide from the Chinese Scorpion Buthus martensi Karsch

作　　者：李医明[1] 吴宫[1] 张乃霞[1] 吴厚铭[1] 

机构地区：[1]中国科学院上海有机化学研究所,上海200032

出　　处：《生物化学与生物物理学报》2000年第4期351-355,共5页

基　　金：国家自然科学基金资助项目!(No .2 9673 0 5 5 );国家科技部资助;中国科学院基金!KJ951 A1 50 4 0 1的经费资助&&

摘　　要：对马氏钳蝎粗毒中多肽进行分离纯化及鉴定。应用凝胶过滤、离子交换和HPLC反相色谱方法分离纯化样品。应用二巯基苏糖醇 (DTT)还原 ,碘乙酸保护法对纯化样品进行修饰 ,修饰样品经酶解后应用Edman降解法 ,并结合ESI MS法和串联质谱法 (MS MS)分析肽的序列。比较修饰前后的质谱 ,测定分子中的二硫键数目。分离纯化获得多肽BmK4112 ,其一级结构为TPYPVNCKTDRDCVMCGLGISCKNGYCTGQC。分子中含三对二硫键。经搜寻蛋白质序列库 ,BmK4112为一新的多肽。To purify and characterize peptides from the venom of Chinese scorpion Buthus martensi Karsch, the purification was carried out by gel filtration, ion exchange and reversed phase HPLC techniques. The purified peptide was reduced by dithioerythritol (DTT), S alkylated with iodoacetic acid, and subjected to enzymatic cleavages (TPCK trypsin). The purified fragments from enzymatic cleavage of the peptide were separated by C 18 HPLC, then submitted to the ESI MS, and Edman degradation for amino acid sequence determination. The mixture was also subjected to tandem mass (MS MS) analysis. As a result, a novel peptide, named BmK4112, was obtained, with the primary structure being: TPYPV NCKTD RDCVM CGLGI SCKNG YCTGQ C, and having three disulfide bonds.

关 键 词：马氏钳蝎 BmK4112 串联质谱 序列分析 分离 

分 类 号：Q516[生物学—生物化学]