Fabrication IL-1Ra loaded galactosylated chitosan nanoparticles for liver targeting  

肝靶向白介素1受体拮抗剂乳糖酰基壳聚糖纳米颗粒的制备(英文)

作　　者：张玲[1,2] 檀家俊[3] 施晓雷[3] 徐师[1,2] 许茜[1,2] 

机构地区：[1]东南大学环境医药及工程国家重点实验室,南京210009 [2]东南大学生物电子学国家重点实验室,南京210096 [3]南京大学医学院附属鼓楼医院肝胆外科,南京210008

出　　处：《Journal of Southeast University(English Edition)》2012年第4期469-473,共5页东南大学学报（英文版）

基　　金：The National Natural Science Foundation of China(No.30901431);the Natural Science Foundation of Jiangsu Province(No.BK2010242)

摘　　要：Galactosylated chitosan （GC） is synthesized and used to prepare IL-1Ra loaded GC nanoparticles by an electrospraying technique. Polyethylene oxide （PEO） is mixed with GC to enhance the electrospraying ability. The effect of the spraying solution properties on particle formation is investigated. The IL-1Ra loaded nanoparticles with an average diameter of 530 nm and a regularly spherical shape are observed by the scanning electron microscopy （SEM）. The amount of the IL-1Ra is measured by the enzyme-linked immunosorbent assay （ELISA） kit. The loading capacity of the nanoparticle is （1.52± 0.04）% （n = 3） and the encapsulation efficiency reaches （90. 36 ± 3.46） % （n = 3）. For the evaluation of GC nanoparticles＇ hepatocytes targeting efficacy, hepatocytes and mesenchymal stem cells （MSCs） are incubated with FITC-labeled GC nanoparticles for 24 h as the experimental and control groups. Results of the fluorescence microscope show that the fluorescence signals observed in hepatocytes are significantly higher than in the MSCs, indicating that the developed GC nanoparticles have an obvious liver targeting property.合成乳糖酰基壳聚糖(GC)并以此为载体材料,采用静电喷射法制备载白介素1受体拮抗剂(IL-1Ra)GC纳米颗粒.在实验过程中,加入一定量聚氧化乙烯(PEO)于GC电纺溶液中,考察电纺溶液性质对颗粒形成的影响,优化溶液性质参数,通过扫描电子显微镜(SEM)观察得到表面光滑、颗粒平均直径约为530nm的载IL-1Ra纳米颗粒,并通过酶联免疫(ELISA)试剂盒检测得到载药纳米颗粒包封率为(1.52±0.04)%(n=3),载药率达到(90.36±3.46)%(n=3).为了考察所制备的GC纳米颗粒对肝细胞的亲和靶向性,实验以猪肝细胞为实验组,猪骨髓基质干细胞为实验对照组,分别加入FITC标记的GC纳米颗粒培养24h,荧光显微镜下观察到猪肝细胞表面荧光信号明显强于骨髓基质干细胞,表明本实验所制备的GC纳米颗粒具有明显的肝靶向功能.

关 键 词：NANOPARTICLE galactosylated chitosan ELECTROSPRAYING liver targeting 

分 类 号：TQ31[化学工程—高聚物工业]