葡萄糖酸氯己定对两种多药耐药菌生物膜的实验研究  

Gluconic acid chlorhexidine experiments on two types of multidrug-resistant bacteria biofilms

作　　者：顾晓红 任慧[2] 洪怡 梁斌 

机构地区：[1]上海市中医药大学附属市中医医院感染管理科,上海200071 [2]上海市闸北区疾病预防控制中心病媒消毒科,上海200070

出　　处：《中华医院感染学杂志》2013年第1期1-3,共3页Chinese Journal of Nosocomiology

基　　金：2011年度中华医院感染控制研究基金资助项目(004)

摘　　要：目的研究多药耐药菌生物膜对葡萄糖酸氯己定的抗力。方法应用滤纸膜片法制备金黄色葡萄球菌和铜绿假单胞菌两种多药耐药菌不同龄期的生物膜;分别采用2%葡萄糖氯己定皮肤消毒剂和2%葡萄糖酸氯己定醇皮肤消毒剂,按载体定量试验方法,测定其对两种多药耐药菌生物膜的最低杀菌浓度。结果两种消毒剂对2d生物膜的最低杀菌浓度为2500～10 000mg/L;对5d生物膜的最低杀菌浓度为5000～10 000mg/L;铜绿假单胞菌生物膜对葡萄糖酸氯己定的抗力要强于金黄色葡萄球菌生物膜;5d生物膜抗力要强于2d生物膜。结论葡萄糖酸氯己定对多药耐药菌细菌生物膜有较理想清除和杀灭作用。OBJECTIVE To study the resistance of muhidrug-resistant bacteria biofilm to gluconic acid chlorhexidine. METHODS The application of filter paper diaphragm was prepared BBF two different periods of the muhidrug-resistant organism（MDROs） clinical Staphylococcus aureus and Pseudomonas aeruginosa. With the 2% glucose chlorhexidine skin disinfectant and chlorhexidine gluconate set alcohol skin disinfectant, the minimum bactericidal concentration of MDROs biofilms was tested by quantitative germicidal test. RESULTS Two kinds of gluconic acid chlorhexidine disinfectant MBC made the minimal bactericidal concentration of 2500-10 000 mg/L within two days , and 5000-10 000 mg/L within 5 days. The resistance of P. aeruginosa was stronger than the S. aureus to gluconic acid chlorhexidine, and the resistance of the biofilms formed by 5 days was stronger than that of those formed within 2 days. CONCLUSION The gluconic acid chlorhexidine is an ideal disinfectant for the clearance and killing of the multidrug-resistant bacteria biofilms.

关 键 词：氯己定 生物膜 最低杀菌浓度 

分 类 号：R187.2[医药卫生—流行病学]