姜黄素衍生物FM0817体外抗多药耐药铜绿假单胞菌作用  

In vitro antibacterial activity of curcumin derivative FM0817 against multidrug-resistant Pseudomonas aeruginosa

作　　者：孙各琴[1] 彭祖旺[1] 胡文波[1] 杨山虹[1] 梁培松[1] 卢兰芬[1] 吴秀娟[1] 张秀明[1] 

机构地区：[1]中山大学附属中山市人民医院检验医学中心,广东中山528400

出　　处：《中华医院感染学杂志》2013年第1期8-10,共3页Chinese Journal of Nosocomiology

基　　金：中山市科技计划项目(20113A053)

摘　　要：目的探讨姜黄素衍生物FM0817体外抗多药耐药铜绿假单胞菌作用及其机制。方法应用VITEK-2Compact系统检测25株多药耐药性铜绿假单胞菌对抗菌药物的敏感性、β-内酰胺酶类和氨基糖苷类药物耐药表型,PCR扩增相应耐药基因;滤纸片扩散法判断FM0817抗菌作用,结合耐药表型和基因型分析FM0817体外抗铜绿假单胞菌作用机制。结果 25株铜绿假单胞菌耐药表型和基因检测结果:β-内酰胺类耐药表型4种;阳性耐药基因9种:主要有blaSHV 20%和blaoprD2基因缺失率60.00%;氨基糖苷类耐药表型4种;阳性耐药基因4种:主要有aac(6′)-Ⅰ80%,ant(3″)-Ⅰ32%;FM0817对25株铜绿假单胞菌抑菌环直径均在9～13mm,无明显差异。结论 FM0817有较强的体外抗铜绿假单胞菌作用,有进一步研发的前景;其作用机制与多药耐药性铜绿假单胞菌耐药表型和基因型未发现有关系。OBJECTIVE To analyze the in vitro antibacterial activity of curcumin derivative FM0817 and its mechanism to multidrug-resistant Pseudomonas aeruginosa. METHODS The drug susceptibility and the drug resistance phenotypes to β-lactamases and aminoglycoside of 25 strains of multidrug-resistant P. aeruginosa were tested by using the VITEK-2 Compact. PCR was used to amplify the relevant drug resistant genes; the antibacterial activity of FM0817 was judged by disc diffusion method, the in vitro antibacterial mechanism of FM0817 on P. aeruginosa was analyzed according to the drug resistant phenotypes combined with genotyping. RESULTS There were 4： 13-1actamase drug-resistant phenotypes in 25 strains of P. aeruginosa ;9 genotypes of drug resistant genes were tested positive, mainly including bla shy 20% and the rate of oprD2 gene deletion was 60.00%. There were 4 aminoglycosides-resistant phenotypes, 4 genotypes of drug resistant genes were tested positive, mainly including aac （6′）- Ⅰ （80 %） and ant （3＂）- Ⅰ （32%）. The bacteriostatic annulus diameters of FM0817 to 25 strains of P. aeruginosa ranged from 9mm to 13mm, the difference was not significant. CONCLUSION FM0817 exhibits Potent in vitro antibacterial activity against P. aeruginosa and deserves further research and development. It does not find any relationship between the mechanism of FM0817 and the drug resistant phenotypes and genotypes of the multidrug-resistant P. aeruginosa.

关 键 词：姜黄素 衍生物 耐药基因 耐药表型 

分 类 号：R378.991[医药卫生—病原生物学]