不同消毒方法对试验操作台HBV-DNA清除方法比较  

Different disinfection methods to clear HBV-DNA on bench:a comparative study

作　　者：刘军权[1] 周燏[1] 

机构地区：[1]解放军第97医院实验科,江苏徐州221004

出　　处：《中华医院感染学杂志》2013年第1期121-123,共3页Chinese Journal of Nosocomiology

摘　　要：目的对试验操作台HBV-DNA清除方法进行比较,以选择最佳的清毒方法。方法将高浓度(5×107拷贝/ml)、中浓度(5×105拷贝/ml)和低浓度(5×103拷贝/ml)HBV-DNA阳性血清标本分别按16cm2/ml比例刷于试验操作平台,制成污染面;用紫外线照射消毒、"84"消毒液、过氧乙酸消毒液、次氯酸钠消毒液和2%(v/v)戊二醛消毒液按常规方法对被污染操作台进行消毒处理;收集经消毒后的标本用PCR检测技术检测HBV-DNA浓度的变化。结果不同的紫外线照射时间对污染台面不同浓度的HBV-DNA清除率有明显差异,照射时间越长,清除率越高,以照射2h后对低浓度的HBV-DNA污染标本清除率最高,明显高于对照组,两组比较差异有统计学意义(P<0.05);中、低浓度BV-DNA污染标本"84"消毒液能完全清除,并能显著降低高浓度污染标本中HBV-DNA含量;次氯酸钠消毒液消毒后也能明显减低高、中和低浓度污染标本中的HBV-DNA含量,与消毒前比较差异有统计学意义(P<0.05);过氧乙酸、次氯酸钠、戊二醛消毒液能减少HBV-DNA数量。结论 "84"消毒液能完全清除中、低浓度的HBV-DNA污染物;过氧乙酸、次氯酸钠和2%(v/v)戊二醛及紫外线照射消毒法不能完全清除HBV-DNA阳性血清污染物中的HBV-DNA。OBJECTIVE To compare the different disinfection methods to clear the HBV-DNA on the bench so as to choose the best disinfection method. METHODS Three different concentrations of HBV-DNA positive serum （5×10^7copies/ml, 5×10^5 copies/ml, 5×10^3 copies/ml） were brushed on the bench at the ratio of 16 cm^2/ml, respectively, the contaminated surface was made. The surface was sterilized with common ways including UV, 84 disinfectant, peracetic acid disinfectant, sodium hypochlorite disinfectant and 2% （v/v）glutaraldehyde disinfectant. The samples were collected after disinfection and the HBV-DNA was detected with PCR. RESULTS There was a significant difference in the clearance rate of HBV-DNA between the various duration of UV irradiation. The longer was the exposure time, the higher was the clearance rate. The clearance rate of low-concentration contaminated HBV-DNA samples was the highest 2 hours after the irladiation, significantly higher than the control group, the difference was statistically significant （P〈0. 05）. The 84 disinfectant could completely clear the low and medium concentrations of HBV-DNA contaminated specimens and could also significantly reduce the content of HBV-DNA in the high concentrations of contaminated specimens. Sodium hypochlorite disinfectant could also significantly decrease the HBV-DNA content in the high, middle and low concentrations of contaminated samples, the difference was statistically significant as compared with that before the disinfection （P〈0.05）. Peracetic acid, sodium hypochlorite, and glutaraldehyde disinfectant could all reduce the HBV-DNA content. CONCLUSION The 84 disinfectant can clear middle and low concentration of HBV-DNA completely. UV, peracetic acid disinfectant, sodium hypochlorite disinfectant and 2M （v/v） glutaraldehyde disinfectant can not completely clear HBV-DNA in the contaminants in HBV-DNA-positive serum.

关 键 词：乙型肝炎病毒-DNA 操作台 聚合酶链反应 

分 类 号：R187[医药卫生—流行病学]