母源性十溴联苯醚暴露对仔鼠海马胆碱能神经递质系统的影响  

Eeffect of maternal BDE-209 on the cholinergic neurotransmitter system in the hippocampus of offspring rats

作　　者：吴英[1] 余艳红[1] 陈敦金[2,3] 

机构地区：[1]南方医科大学南方医院妇产科,广州市510515 [2]广州医学院第三附属医院妇产科 [3]广州市孕产妇重症救治中心,510150

出　　处：《实用医学杂志》2013年第1期34-36,共3页The Journal of Practical Medicine

基　　金：国家重点自然科学基金资助项目(编号:40590390)

摘　　要：目的:从胆碱能神经递质系统角度探讨母源性十溴联苯醚(BDE-209)损伤仔鼠神经系统的可能机制。方法:3月龄wistar雌鼠自确定交配成功后随机分为实验组(A、B、C、D组)和对照组(E组),通过灌胃方法建立自妊娠期至哺乳期的不同剂量BDE-209[100、300、600、1200mg/(kg.d)]的暴露模型,21d仔鼠断乳后各组随机选取10只仔鼠作为研究对象,应用免疫组织化学和比色法分别测定各组仔鼠海马组织中乙酰胆碱纤维(ACH)含量、乙酰胆碱酯酶(ACHE)活性、毒蕈碱受体(M-ACHR)含量、烟碱受体(N-ACHR)含量。结果:与E组相比,B、C、D组ACH含量和ACHE活性下降;C、D组M-ACHR含量下降;仅D组N-ACHR含量下降。结论:一定剂量的母源性BDE-209可能通过影响仔鼠胆碱能神经递质系统而具有神经毒性,且存在剂量效应关系。Objective To study the possible mechanism of the danlage of nerve system of prenatal induced by lactational BDE-209 （brominated Diphenyl Ethers-209） in the offspring rats from the cholinergic neurotransmitter system point of view. Methods After mating successfully, the female Wistar rats at the age of 3 months were divided into five groups randomly including the experimental group A, B, C, D and the control group E. BDE-209 was given in doses of 100（group A）, 300（group B）, 600（group C）, 1 200 （group D） mg/（kg＇d） by oral garage for maternal rats in the experimental groups. Ten rats of the first weaning offspring in each group were selected randomly. We determined the contents of acetylcholine, muscarinic cholinoreceptor, cholinergic nicotinic receptor and the activity of acetylcholinesterase by immunohistochemistry and chromatometry in each group. Results Compared with group E, the concentration of acetylcholine and the activity of acetylcholinesterase decreased significantly in group B, C and D, the concentration of muscarinic cholinoreceptor decreased significantly in group C and D, and the concentration of cholinergic nicotinic receptor decreased significantly in group D. Conclusions A certain dose of maternal BDE-209 （deca-BDE） could be neurotoxical due to the effect on the cholinergic neurotransmitter in the offspring rats, and there was dose-effect.

关 键 词：十溴联苯醚 BDE-209 海马 乙酰胆碱纤维 乙酰胆碱脂酶 受体 

分 类 号：R114[医药卫生—卫生毒理学]