结晶紫的酶催化共振散射光谱法测定痕量过氧化氢  

Enzyme catalytic determination of trace hydrogen peroxide using crystal violet as resonance light scattering spectral probe

作　　者：蒙华毅[1] 李欣[1] 覃温露[1] 唐宁莉[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,桂林541004

出　　处：《分析试验室》2013年第1期16-18,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21165008)资助

摘　　要：在pH4.7的HAc-NaAc缓冲溶液中,牛血红蛋白可催化H2O2氧化I-生成I2,I2与过量的I-生成的I3-与带正电荷的结晶紫(CV+)可形成缔合物微粒,导致体系的共振散射光强度增强。在659 nm处,H2O2在2.065×10-7～1.652×10-6mol/L范围内与共振散射光强度的增加值(ΔI)呈良好的线性关系,相关系数r为0.9989,检出限为2.676×10-9mol/L。据此,建立了检测痕量H2O2的共振散射光谱新方法,该方法已用于水样中H2O2含量的测定。In pH 4.7 HAc-NaAc buffer solution,based on the catalytic effect of bovine hemoglobin,hydrogen peroxide oxidized the excess I-into I2,I2 reacted with the excess I-to form I-3,and I3-combined with crystal violet（CV＋） to form association particles,which enhanced the resonance light scattering intensity greatly.And the increased resonance light scattering value（ΔI） was linear to the concentration of hydrogen peroxide in the range of 0.207-1.65 μmol/L,whereby a new method for the determination of hydrogen peroxide by resonance light scattering（RLS） method was established.The correlation coefficient was 0.9989,and the detection limit was 2.68 nmol/L.This method has been applied to the determination of hydrogen peroxide concentration in water samples with satisfactory results.

关 键 词：牛血红蛋白 过氧化氢 结晶紫 共振散射 

分 类 号：O657.3[理学—分析化学]