荧光探针法快速测定牛奶中三聚氰胺  

Rapid determination of melamine content in milk by fluorescent probe assay

作　　者：程定玺[1] 黄世江[1] 梁宇[1,2] 王超海[2] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007 [2]新乡学院化学与化工学院,新乡453003

出　　处：《分析试验室》2013年第1期48-51,共4页Chinese Journal of Analysis Laboratory

基　　金：河南省重点科技攻关项目(102102210043)资助

摘　　要：在pH 8.40的缓冲溶液中,一定浓度的溴化十六烷基三甲基铵可以使阴离子染料荧光素的荧光强度显著增强,加入一定量的三聚氰胺后,其荧光发生猝灭,且荧光猝灭的程度在一定的范围内与加入的三聚氰胺的量呈良好的线性关系,据此建立了以荧光素作为荧光探针测定三聚氰胺含量的荧光光谱法。优化了实验条件,获得的线性范围为0.025～0.85 mg/L,检出限为0.0019 mg/L。样品回收率为82.9%～87.4%,相对标准偏差为1.9%～4.0%。方法可用于牛奶中三聚氰胺的快速测定。In the Sфrensen buffer solution at pH 8.40,the fluorescence intensity of anionic dye fluorescence（F） is significantly enhanced after added a certain concentration of the cationic surfactant cetyltrimethylammonium bromide（CTMAB）.It was found that melamine（MEL） had the ability to quench the fluorescence,and the quenched extent was proportional to the concentration of melamine within a certain range.Based on the experimental results,a novel fluorescence spectrometry for the determination of melamine was proposed by using fluorenscent probe.The optimum conditions were obtained by single factor experiment.The linear range of this assay is 0.025-0.85 mg/L,the limit of detection is 0.0019 mg/L,the average recoveries are 82.9%-87.4%,and the relative standard deviation are 1.9%-4.0%.The method is simple,quick,selective and sensitive,and is suitable for the determination of melamine content in milk.

关 键 词：荧光探针 荧光素 三聚氰胺 

分 类 号：O657.3[理学—分析化学]