不同切花菊品种及处理对愈伤组织诱导和分化的影响  

Effects of Different Cut Chrysanthemum Culticars and Treatments on Callus Induction and Differentiation

作　　者：时颂[1] 李青[1] 赵霜[1] 戴思兰[1] 李娜娜[1] 

机构地区：[1]北京林业大学,北京100083

出　　处：《东北林业大学学报》2013年第1期77-81,共5页Journal of Northeast Forestry University

基　　金：国家林业局林业公益性行业科研专项(200904050)

摘　　要：以切花菊‘神马’‘雪山’‘雪神’‘粉贵人’‘黄金’‘优香’6个品种的不同取材部位为试材,接种于含不同类型和质量浓度激素的培养基中,并在不同的光照条件下进行培养,研究影响切花菊愈伤组织诱导和分化的主要因素。结果表明:‘粉贵人’的叶片和茎段是最佳的外植体材料;在愈伤组织诱导试验中,2,4-D的效果优于NAA,6-BA的效果优于KT;在1 200 lx的光照条件下,愈伤组织诱导率高、增殖速度快,且后期分化能力强。试验还对愈伤组织不同的结构类型进行研究,认为Ⅱ、Ⅲ型愈伤组织,无论是从结构的疏松度、增殖速度还是后期分化能力都明显高于其他类型愈伤组织。Six Dendrathema morifolium cuhivars of ＇ Shenma＇ , ＇ Xueshan＇ , ＇ Xueshen＇ , ＇ Fenguiren＇ , ＇ Huangjin＇ , ＇ Youx- iang＇ under different medium and light treatment were selected to study the induction and differentiation factors of Den- dranthema morifolium callus. The results show that： Leaves and stems of D. morifolium ＇ Fenguiren＇ are the best ex- plants. The most suitable auxin and Cytokinin for callus induction are 2,4-D and 6-BA rather than NAA and KT. Under 1 200 lx light treatment, callus has higher induction efficiency, faster proliferation rate and faster later differentiation rate. In addition, the research results from different structure and type of callus show that Ⅱ and Ⅲ callus were better than others in terms of the structure porosity, the proliferation rate and the later differentiation rate.

关 键 词：切花菊 愈伤组织 诱导 植物生长调节剂 光照培养 

分 类 号：S682.11[农业科学—观赏园艺] Q943.1[农业科学—园艺学]