高效液相色谱法测定虾中的酸性红1、铬变素FB和丽春红2R3种人工合成色素  

Determination of acid red 1,chromotrope FB and xylidine ponceau 2R in shrimp by high performance liquid chromatography

作　　者：王焦清[1] 吴官非 夏焜[1] 曹旭妮[1] 

机构地区：[1]华东理工大学食品系生物反应器工程国家重点实验室,上海200237

出　　处：《化学试剂》2013年第1期37-39,88,共4页Chemical Reagents

基　　金：上海市科委资助项目(11142201200;11142200703;10142200803;11nm0505200);上海市教委资助重点项目(12ZZ048)

摘　　要：为建立食品中非食用和食用色素酸性红1、铬变素FB和丽春红2R的高效液相色谱定量检测方法,虾样品经乙醇-氨水提取,以乙腈-10 mmol/L醋酸铵水溶液(pH 8.0)为流动相,Hydrosphere-C18色谱柱分离,二极管阵列检测器检测,检测波长为504 nm,外标法定量分析了自制浸泡虾皮中的色素含量。该方法线性范围为2.0×10-7～1.0×10-4mol/L,酸性红1、铬变素FB和丽春红2R最低检出浓度分别为7.7×10-8、9.1×10-8和5.9×10-8mol/L;该方法的回收率为95%～107%,相对标准偏差(RSD)为1.5%～2.5%。A method for the determination of acid red 1 ,chro- motrope FB and xylidine ponceau 2R in shrimp was established by high performance liquid chromatography （ HPLC ）. acid red 1,chromotrope FB and xylidine ponceau 2R was extracted by alcohol-ammonia. The separation was performed in aYMC Hydrosphere-C18（250 min×4.6 mm i.d. 5 um） column, acetonitrile and 10 mmol/L ammonium acetate （pH 8.0） were used as mobile phase. A photo-diode array detector was used to detect them at 504 nm. The linear range was 2.0 ×10^-7 - 1.0×10^-4 mol/L,and the detection limit of acid red 1, chromotrope FB and xylidine ponceau 2R was 7.7 ×10^-8,9.1×10^-8 and 5.9 ×10^-8 ,respectively. The re- coveries ranged from 95% to 107% and RSD was 1.5% - 2.5%.

关 键 词：酸性红1 铬变素FB 丽春红2R 高效液相色谱 虾 

分 类 号：O657.7[理学—分析化学]