葛根素对神经病理性痛模型小鼠的镇痛作用  

The analgesic effect of puerarin on neuropathic pain mice

作　　者：罗敬华[1,2] 曾晓艳[3] 范桂香[1] 袁育康[1] 王军阳[1] 

机构地区：[1]西安交通大学医学院免疫学与病原生物学系,西安710061 [2]西安市北车医院检验科,西安710086 [3]西安交通大学医学院第一附属医院检验科,西安710061

出　　处：《西北药学杂志》2013年第1期48-50,共3页Northwest Pharmaceutical Journal

摘　　要：目的探讨葛根素对神经病理性痛模型小鼠的镇痛作用,为临床开发新的镇痛药物奠定基础。方法结扎雌性C57BL/6小鼠单侧胫神经和腓总神经,建立坐骨神经分支选择损伤(spared nerve injury,SNI)神经病理性痛模型,利用机械刺激法和冷盘法分别观察腹腔注射不同剂量葛根素(100,75和25mg·kg-1)对SNI模型小鼠患侧脚掌痛阈的影响。结果 SNI模型小鼠腹腔注射75mg·kg-1葛根素可显著提高患侧脚掌的50%缩足阈值(P<0.01)和降低5min抬足次数(P<0.01),产生明显的镇痛作用,镇痛时间可维持60～70min;100mg·kg-1葛根素腹腔注射后虽然也可产生明显的镇痛作用,但作用时间较短,仅维持10～20min;25mg·kg-1葛根素腹腔注射后没有明显的镇痛作用。结论适当剂量的葛根素对神经病理性痛具有明显的镇痛作用。Objective To investigate the analgesic effect of puerarin on the neuropathic pain,and screen novel analgesics for clinical treatment of neuropathic pain diseases.Method The neuropathic pain was induced by tightly ligating the unilateral tibial and common peroneal nerve of mouse with leaving the sural nerve intact,and the pain thresholds before and after intraperitoneal injection of the different doses of puerarin（100,75 and 25 mg · kg-1） were measured by the mechanical and cold stimulus.Results 75 mg · kg-1 of puerarin significantly increased the 50% withdrawal threshold（P0.01） and decreased the paw lifting times in 5 min（P0.01） of mice with spared nerve injury（SNI）,the analgesic effect lasted 60-70 min;100 mg · kg-1 of puerarin also produced significant analgesic effect,but relative shorter time,only lasted 10-20 min;puerarin at dose of 25 mg · kg-1 did not show any analgesic effect.Conclusion Suitable dose of puerarin has obvious analgesic effect on neuropathic pain.

关 键 词：葛根素 神经病理性痛 镇痛 

分 类 号：R965[医药卫生—药理学]