氟吡菌胺与吡唑醚菌酯混合物对黄瓜霜霉病菌的毒力增效及其抗药性的影响  

Synergistic Interaction and Effects of Mixture of Fluopicolide with Pyraclostrobin on Resistance of Pseudoperonospora cubensis to Fungicides

作　　者：闫磊[1,2,3] 王文桥[2,3] 孟润杰[2,3] 赵建江[2,3] 韩秀英[2,3] 马志强[2,3] 张小风[2,3] 张金林[1] 

机构地区：[1]河北农业大学植物保护学院农药系,河北保定071000 [2]河北省农林科学院植物保护研究所,河北保定071000 [3]农业部华北北部作物有害生物综合治理重点实验室,河北保定071000

出　　处：《农药》2013年第1期53-56,共4页Agrochemicals

基　　金：公益性行业(农业)科研专项(201003004);农林生物灾害防控关键技术研究与示范(2012BAD19B06)

摘　　要：[方法]采用植株喷雾-叶盘法测定氟吡菌胺与吡唑醚菌酯混合物对黄瓜霜霉病菌的联合毒力,采用叶盘漂浮法测定黄瓜霜霉病菌经氟吡菌胺、霜霉威、吡唑醚菌酯、氟吡菌胺+吡唑醚菌酯(1:4)和氟吡菌胺+霜霉威(1:10)连续驯化3、5、7代后对氟吡菌胺、霜霉威和吡唑醚菌酯的敏感性。[结果]氟吡菌胺与吡唑醚菌酯以(5:1)、(3:1)、(1:1～7)混合对黄瓜霜霉病菌的毒力表现增效。黄瓜霜霉病菌经氟吡菌胺与霜霉威混合物或与吡唑醚菌酯混合物连续驯化后的抗药性均比经3种单剂连续驯化后的抗药性发展缓慢,黄瓜霜霉病菌经2种混合药剂连续驯化7代获得的抗性菌株对氟吡菌胺的抗性水平为12.0～13.4倍,对吡唑醚菌酯和霜霉威的抗性水平分别为9.6、4.2倍,而经氟吡菌胺、吡唑醚菌酯和霜霉威3种单剂驯化7代获得的抗性菌株对氟吡菌胺、吡唑醚菌酯和霜霉威抗性水平分别为16.3、11.6、5.1倍,均高于混合药剂驯化所得抗性菌株的抗性水平。[结论]在氟吡菌胺与吡唑醚菌酯9个配比混合物中,1:4混合物对黄瓜霜霉病菌的毒力增效最明显;氟吡菌胺与吡唑醚菌酯1:4混合物及其与霜霉威1:10混合物的使用可延缓黄瓜霜霉病菌对氟吡菌胺抗性的发展。[Methods] The joint-toxicity of the mixture of fluopicolide with pyraclostrobin against Pseudoperonospora cubensis was determined by potted plant spray-leaf disc test. P. cubensis was tamed for seven generations by using fluopicolide, pyraclostrobin, propamocarb hydrochloride and two kinds of mixtures, respectively. The sensitivity of 3, 5, 7th generations of tamed P. cubensis to fluopicolide, pyraclostrobin and propamocarb hydrochloride were tested by leaf disc floating method. [Results] The results showed that the mixtures of 5 ： 1, 3 ： 1, 1 ： （1-7） exhibited synergistic interaction. P. cubensis tamed with mixture of fluopicolide and pyraclostrobin or propamocarb hydrochloride developed resistance more slowly than tamed with three single fungicides. The resistance factors of the mutants acquired by seven subcultures of P. cubensis on the leaf discs treated with two kinds of mixtures to fluopicolide, pyraclostrobin and propamocarb hydrochloride were up to 12.0-13.4, 9.6 and 4.2 fold, respectively. However, the mutants acquired by seven subcultures ofP. cubensis on the leaf discs treated with the three single fungicides showed 16.3, 11.6 and 5.1 fold resistance factors. [Conclusions] The results suggested that synergistic interaction against P. cubensis existed in the mixtures of fluopicolide with pyraclostrobin; application of mixture of fluopicolide with fungicides （pyraclostrobin or proparnocarb hydrochloride） of different action mechanisms could delay development of resistance ofP. cubcnsis to fluopicolide.

关 键 词：黄瓜霜霉病菌 氟吡菌胺与吡唑醚菌酯或霜霉威混合物 增效作用 继代培养 抗药性发展 

分 类 号：S482.2[农业科学—农药学]