接种两种固氮菌增强小麦幼苗抗渗透胁迫及生长能力  

Inoculation two azotobacter enhancing osmotic stress resistance and growth in wheat seedling

作　　者：刘华伟[1,2] 林晓军[1,2] 孙超[1,2] 李强[1,2] 杨呼[1,2] 郭蔼光[1,2] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]旱区作物逆境生物学国家重点实验室,陕西杨凌712100

出　　处：《植物生态学报》2013年第1期70-79,共10页Chinese Journal of Plant Ecology

基　　金：国家自然科学基金项目(30700489和31071870);陕西省科学技术研究发展计划项目(2010K02-12-2);西北农林科技大学基本科研业务费专项资金项目(QN2009067)

摘　　要：苗期是小麦(Triticum aestivum)物质和能量积累的关键时期,苗期干旱影响小麦的后期群体建成。利用田菁茎瘤固氮根瘤菌(Azorhizobium caulinodans)‘ORS571’与巴西固氮螺菌(Azospirillum brasilense)‘Yu62’浸种侵染小麦和聚乙二醇(PEG)模拟渗透胁迫,研究渗透胁迫下接菌小麦种子的发芽状况;利用固氮菌涂抹小麦幼苗叶部,测定PEG模拟渗透胁迫下小麦幼苗根体积、叶片相对含水量、脯氨酸含量及可溶性蛋白含量,探究固氮菌增强小麦幼苗抗渗透胁迫的能力。结果表明,接种混合固氮菌后在渗透胁迫下小麦种子的发芽率明显提高;在渗透胁迫下叶部涂抹固氮菌小麦的根体积、叶片相对含水量、脯氨酸含量及可溶性蛋白含量明显升高,表明接种固氮菌可提高小麦幼苗的抗渗透胁迫能力,且混合固氮菌对小麦幼苗叶片的增强效果优于单一固氮菌。Aims The seedling stage is the key stage of matter and energy accumulation in the wheat life cycle. Therefore, drought during the seedling stage affects population formation in late stages. In this study, wheat seedlings were inoculated with azotobacters Azorhizobium caulinodans ＇ORS571＇ and Azospirillum brasilense ＇Yu62＇. Methods Wheat seedlings germination was screened in normal conditions and with PEG 6000 osmotic stress using seedlings inoculated with azotobacters. Root volume, relative water content （RWC）, proline content and soluble protein content of seedling laminas were determined under PEG drought stress using seedlings inoculated with azotobacters on laminas. Important findings The germination rate of wheat seedlings was significantly increased under drought stress when inoculated with azotobacters. Moreover, wheat seedlings inoculated with mixed azotobacters have more obvious growth promotion than when inoculated with a single azotobacter. The former laminas proline content, relative water content, proline content and soluble protein content had increased. The results showed that drought resistance of wheat seedlings was improved when inoculated with mixed azotobacters, which provided the foun- dation for further study of azotobacter-wheat interaction under drought stress.

关 键 词：抗旱性 混合固氮菌 小麦幼苗 

分 类 号：S512.1[农业科学—作物学]