补充烟酸及耐力训练对急性酒精性肝损伤大鼠SirT1活性与表达的影响及机制研究  

Influence of niacin supplement and endurance training on activity and expression of SirT1 and its mechanism in alcohol-induced acute hepatic injury in rats

作　　者：宋信勇[1] 

机构地区：[1]山东体育学院,山东济南250102

出　　处：《山东体育学院学报》2012年第6期68-72,共5页Journal of Shandong Sport University

摘　　要：目的:研究补充烟酸及耐力训练对急性酒精性肝损伤大鼠SirT1表达、活性的影响及机制。研究方法:以Wistar大鼠建立急性酒精性肝损伤模型,以跑台运动为训练手段,并且给予大鼠补充烟酸,测定血清ALT和AS以及肝脏MnSOD活性、NAD+/NADH比值、MDA含量、SirT1活性、线粒体ATP合成活力、MnSOD和SirT1mRNA表达。结果:急性酒精摄入导致血清ALT和AST水平、肝脏MDA含量均显著升高,线粒体ATP合成活力、肝脏NAD+/NADH比值、MnSOD及SirT1活性及mRNA表达均显著降低;耐力训练及补充烟酸再给予酒精摄入与只给予酒精摄入血清ALT和AST水平、肝脏MDA含量、线粒体ATP合成活力、肝脏NAD+/NADH比值、MnSOD及SirT1活性及mRNA表达变化规律一致,但变化幅度相对较小。结论:耐力训练及补充烟酸均能提高肝脏细胞内NAD+/NADH的比值,使SirT1活性及表达升高,进而提高肝脏细胞抗氧化能力,达到预防/改善急性酒精性肝损伤的目的。Objective：To investigate the influence of niacin supplement and endurance training on the activity and expression of SirT1 and its mechanism in alcohol - induced acute hepatic injury in rats. Methods：It analyzed the level of ALT and AST in serum, NAD ＋/NADH, content of MDA, mito- chondrial ATP synthesis, activities and expressions of MnSOD and SirT1 in liver tissue in alcohol - induced acute hepatic injury rats after treadmill training and niacin supplement. Results： The acute alcohol treatment increases ALT and AST in serum and content of MDA in liver tissue, decreases mitochondrial ATP synthesis, ratio of NAD ~/NADH, activities and expression of MnSOD and SirT1 in alcohol - induced acute hepatic injury rats. The parameters of the above indicates the same after endurance training and niacin supplement but the extent is relatively lower. Conclusion：Endurance training and niacin supplement can increase ration of NAD ＋/NADH, which improves the activity and expression of SirT1, and then increases antioxidant ability of liver, which achieves the goal of protecting or improving alcoholinduced acute hepatic injury.

关 键 词：烟酸 耐力训练 急性酒精性肝损伤 沉默信息调节因子 

分 类 号：G804.7[文化科学—运动人体科学]