双齿围沙蚕Hsp70 cDNA基因的克隆及序列分析  

Cloning and analysis of Hsp70 cDNA from Perinereis aibuhitensis (Annelida:Polychaeta)

作　　者：李颖[1] 周一兵[1] 万良[1] 赵欢[1] 杨大佐[1] 

机构地区：[1]大连海洋大学辽宁省海洋生物资源恢复与生境修复重点实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2012年第6期502-507,共6页Journal of Dalian Ocean University

基　　金：国家"863"计划项目(2006AA10Z410);国家海洋公益性行业科研专项(200805069)

摘　　要：根据已知巨型管状虫Riftia pachyptila热休克蛋白70(Hsp70)基因序列设计引物,应用同源克隆策略和RACE技术首次从双齿围沙蚕Perinereis aibuhitensis体壁肌肉中克隆获得Hsp70 cDNA序列。结果表明:双齿围沙蚕Hsp70 cDNA序列全长为2 336 bp,包括5'端非翻译区88 bp、3'端非翻译区286 bp和开放阅读框1 962 bp;整个开放阅读框编码653个氨基酸,包含Hsp70家族3个特征区域;通过生物信息学分析表明,该蛋白是一种无信号肽跨膜蛋白,理论等电点为5.14,相对分子质量为71 396,半胱氨酸(Cys)含量为0.8%;同源性分析表明,双齿围沙蚕Hsp70氨基酸序列与其他真核生物的Hsp70序列具有很高的相似性。该研究结果可为进一步开发沙蚕Hsp70作为监测海洋污染的一种生物标记物提供重要的参考依据。In present study, the partial eDNA of Hsp70 was amplified from the total RNA of epidermis muscle in polychaete ,Perinereis aibuhitensis by homology cloning strategy and RACE technology, using specific primers based on the reported Riftia pachyptila Hsp70 gene sequence. Sequence analysis revealed that there was a 2 336 bp cDNA containing a 88 bp 5＇-untranslated region, a 286 bp 3＇-untranslated region and 1 962 bp open reading frame enco- ding 653 amino acids. Three conserved motifs of hsp70 family were found in this sequence. The bioinformatic anal- ysis indicated that the deduced amino acid is a non-transmembrane protein with no signal peptide, with isoelectric point of 5.14 and molecular weight of 71 396 and 0.8% of cysteine（Cys） content. The sequence similarity analysis revealed that the deduced amino acid had high homology to other organisms. The findings will provide insight for i- dentifying hsp70 as biomarker of environmental pollution.

关 键 词：双齿围沙蚕 热休克蛋白70 基因分析 

分 类 号：Q78[生物学—分子生物学]