产左聚糖枯草芽孢杆菌的突变选育及多糖产物的生物物理分析  

Mutagenesis Breeding of Levan-producing Bacillus subtilis and Biophysical Analysis of Polysaccharides

作　　者：杨辉[1] 李杰民 余磊[1] 赖祯冬[1] 杜丽琴[1] 韦宇拓[1] 黄日波[3] 

机构地区：[1]广西大学生命科学与技术学院,广西亚热带生物资源保护利用重点实验室,南宁530004 [2]广西农业科学研究院,南宁530007 [3]广西科学院国家非粮生物质能源工程技术研究中心,南宁530007

出　　处：《基因组学与应用生物学》2012年第6期567-573,共7页Genomics and Applied Biology

基　　金：国家自然科学基金(31060125)资助

摘　　要：本研究对多糖产生菌株sp.54A-42进行了16SrDNA序列比对分析,鉴定其为枯草芽孢杆菌。对发酵所得多糖产物进行了薄层层析、高效液相色谱、红外光谱和核磁共振分析,证明发酵产物为左聚糖(β-2,6-果聚糖)。通过紫外-LiCl复合诱变方式选育,得到突变株sp.Z-49,多糖产量达到16.1g/L,比出发菌株提高了34.2%,经5次传代发酵实验产量稳定,为进一步研究左聚糖的发酵生产打下了良好基础。The polysaccharides-producing strain sp.54A-42 was identified to be Bacillus subtilis based on deter-mination and alignment of its 16S rDNA sequence. Its fermenting polysaccharides were proved to be levan （β-2,6-fructan） through the biophysical analysis of thin layer chromatography, high performance liquid chro-matography, infrared spectroscopy and nuclear magnetic resonance. By using the approach of ultraviolet-LiCl compound mutagenesis, the mutant sp.Z-49 was obtained, and the polysaccharides yield of this mutant reached 16.1 g/L with increase of 34.2% compared with the starting strain, which remains a stable yield after five inoculm passages. This research may lay a good foundation for further study on levan producing by fermentation.

关 键 词：枯草芽孢杆菌 左聚糖 突变选育 生物物理鉴定 

分 类 号：S54[农业科学—作物学]