农杆菌介导DR1172基因转化拟南芥  

Preliminary Study on Agrobacterium-mediated Transformation of DR1172 Gene into Arabidopsis

作　　者：张新[1] 崔广艳[1] 陈翠娜[1] 张思维[1] 代其林[1] 王劲[2] 

机构地区：[1]西南科技大学极端环境生物资源利用实验室,绵阳621000 [2]中国农业科学院生物技术研究所,北京100084

出　　处：《基因组学与应用生物学》2012年第6期582-586,共5页Genomics and Applied Biology

基　　金：转基因生物新品种培育重大专项(2009ZX08009-091B);国家自然科学基金(30871555);教育部新世纪优秀人才支持计划(NCET-08-0940);四川省教育厅(09ZA034);西南科技大学博士研究基金(11zx7104)共同资助

摘　　要：耐辐射奇球菌(D.radiodurans,DR)能在极端胁迫条件下生存,并且拥有一个独特的极端环境抗性基因组而被广泛用于研究。其中DR1172是在耐辐射球菌中分离出的一段基因,与LEA76家族同源,参与植物的抗旱机制。本研究利用基因工程手段以载体pBI121为基础构建植物DR1172-GV3103表达载体,然后利用花序浸染法将目的基因DR1172转入模式植物拟南芥中,T2代得到50株植物,阳性为15棵,转化率为30%。该研究为探讨DR1172基因的功能以及植物抗逆机制提供了丰富的拟南芥材料,而且对利用基因工程技术手段改良植物性状,培育出抗逆性强的植物优良品种有重要的指导价值。DR1172 is a part of gene from D. radiodurans which has high resistance to radiation resistance. DR1172 and LEA76 are actually homologous in some way that they both participate in the drought resistant in plants. In this research, DR1172-GV3103 vector was constructed successfully and the DR1172 gene was inserted into Ara-bidopsis by using the transgenic technique. Transgenic Arabidopsis were identified by the molecular identification PCR. 15 positive plants were obtained from 50 T2 Arabidopsis and its conversion rate was 30%. The researches not only provide abundant plant resources to study the function of DR1172, but also have great important applica-tion value in plant gene engineering technology and cultivate quality variety of plants and agricultural production.

关 键 词：拟南芥 DR1172 载体构建 遗传转化 抗旱 

分 类 号：Q943.2[生物学—植物学]