ITS序列作为DNA条形码在中药鸡骨草中的应用  

ITS Sequence Used as DNA Barcode Applied in Chinese Herb Abrus cantoniensis

作　　者：王晓明[1] 牛宪立[1] 魏妮娜[1] 姬可平[1] 

机构地区：[1]遵义医学院珠海校区,珠海519041

出　　处：《基因组学与应用生物学》2012年第6期603-608,共6页Genomics and Applied Biology

摘　　要：以广西和广东的九种中药鸡骨草（Abrus cantoniensis）为材料,利用改良的 CTAB 法进行总 DNA 的提取,应用通用引物对其 rDNA 的内转录间隔区（ITS）序列进行 PCR 扩增,得到9种植物的 ITS 序列,对所得序列进行分析并申请登录到 GenBank,已获得登录号.结果表明,序列的全长为692~702 bp,其中 ITS2为211~212 bp;中药鸡骨草的混淆品小叶黑面神（Breynia vitis-idaea）和其同科同属植物相思子（Abrus precato-rius）的 ITS2序列与中药鸡骨草差异很大;用遗传距离和建立邻接树这两种方法对序列进行分析,都证明了ITS2序列可以作为豆科植物的 DNA 条形码序列;ITS2序列的种间差异为豆科植物的种间鉴别和建立比较合理的 DNA 条形码体系提供较好的依据.Through the modified CTAB method, we get the DNA from nine different Abrus cantoniensis which grow in Guangdong and Guangxi province. In order to get nine kinds of Abrus cantoniensis ITS sequences, we am plified the rDNA internal transcribed spacer region （ITS） sequences by using universal primers through PCR, as well as registered to GenBank and get the accession number. According to the analysis of the result, the length of the ITS sequences is 692~702 bp, and the length of ITS2 sequences is 211~212 bp. On the other hand, there are great differences among the Breynia vitis-idaea, Abrus precatorius, and Abrus cantoniensis in ITS2 squences. Breynia vitis-idaea is similar with Abrus cantoniensis. Abrus precatorius is the same family and genera with Abrus cantoniensis. Furthermore, both K2P genetic distance calculation and the creation of the NJ tree have proved that the ITS2 se-quences can be used as the DNA barcode sequence of the Fabaceae plants. Thus, the difference of the ITS2 pro-vides a great basis of Fabaceae species identification and the establishment of the DNA barcode system.

关 键 词：中药 鸡骨草 ITS序列 DNA条形码 

分 类 号：S567[农业科学—中草药栽培]