植物PDR蛋白家族的进化分析  

Phylogenetic Analysis of the PDR Protein Family in Plant

作　　者：谢小芳[1] 陈志伟[2] 吴为人[1] 

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]福建农林大学作物科学学院,福州350002

出　　处：《基因组学与应用生物学》2012年第6期617-623,共7页Genomics and Applied Biology

基　　金：福建省自然科学基金项目(2012J01076)资助

摘　　要：植物多向耐药性(pleiotropic drug resistance,PDR)蛋白亚家族是ATP结合盒(ATP-binding cassette,ABC)蛋白家族的一员,它们参与生物体内多种物质的转运,在生物的一些重要生理过程中起关键作用。本研究采用生物信息学的方法,利用植物中已经分离的PDR蛋白为检索序列,在全基因组水平上搜索水稻、莱茵衣藻和小立碗藓的蛋白序列,最终确定了30个PDR候选蛋白,其中水稻15个,莱茵衣藻5个,小立碗藓10个。ESTs表达分析为其中的27个蛋白找到了表达证据。对相应蛋白的结构和基因之间的系统演化关系进行了分析,结果表明:植物的PDR蛋白具有高度保守的基序,并且在蛋白中的排列顺序也大致相同;该蛋白家族在低等的藻类植物中就已经发生了分化,其基本特征已经形成,在高等植物中均以物种特异的方式进行了扩增。内含子/外显子结构分析显示:低等的藻类植物中的PDR蛋白的内含子数目最多,暗示在小立碗藓和水稻等高等植物的进化过程中发生了内含子的丢失事件。The plant pleiotropic drug resistance （PDR） protein subfamily is one of ATP-binding cassette （ABC） protein family members, which involved in transporting many kinds of substrates in living organisms and closely related to some important biological processes. In the present research, taking plant PDR protein which has been isolated as the retrieval sequences, we searched the homologous sequence from Oryza sativa, Chlammydomonas reinhardtii and Physcomitrella patens protein database based on the complete genome by bioinformatics method. 30 PDR-like proteins were identified, 15 were from Oryza sativa, 5 were from Chlammydomonas reinhardtii and 10 were from Physcomitrella patens. 27 proteins found expressing information by ESTs expression analysis. The structure and systematic characterization of 30 PDR-like were performed. The analysis results demonstrated thatthe motifs in the PDR protein were highly conservative, and the order of these motifs in the proteins was quite similar. The phylogenetic analysis of these proteins suggested that divergence of this family should have occurred and the main characteristics should have established in lower plant of algae; species-specific expansion occurred in higher plant. Intron/exon structure analysis indicated that algae should have more numbers of intron than Physcomitrella patens and Oryza sativa, it suggested that intron missing should be an important event accompany-ing the recent evolution of high plant PDR family.

关 键 词：植物 PDR蛋白家族 进化 生物信息学 

分 类 号：Q946.1[生物学—植物学]