南方红豆杉中紫杉醇产生菌的筛选及鉴定  

Screening and Identification of Taxol-Producing Fungus from Taxus Mairei

作　　者：张远都 范志茹[2] 周毅刚[3] 邓桢翰[2] 

机构地区：[1]河南省洛阳市第一高级中学,河南洛阳471000 [2]中南大学湘雅医院,湖南长沙410078 [3]中南大学基础医学院微生物教研室,湖南长沙410013

出　　处：《医学临床研究》2012年第12期2257-2259,共3页Journal of Clinical Research

基　　金：2004年度科技部中国APEC科技产业合作基金项目资助（国科外函[2004]240号）

摘　　要：[目的]从南方红豆杉韧皮部筛选紫杉醇产生菌。[方法]利用无菌技术从南方红豆杉韧皮部分离内生真菌，并通过TLC和HPLC对其培养物中紫杉醇进行分析和鉴定。[结果]从南方红豆杉韧皮部筛选出了20种200余株内生真菌，得到一株高产紫杉醇产生菌，其培养液中紫杉醇含量高达1．003mg／L。[结论]通过对其个体和群体形态进行观察，所分离的紫杉醇产生菌属链格孢属的一个新种。[Objective] To screen the taxol-producing endophytic fungi from Taxus mairei barks. [Methods]The endophytic fungi were isolated from Taxus mairei barks by using aseptic techniques. Taxol in culture medium was analyzed with thin-layer chromatography（TLC） and high performance liquid chromatogra- phy （HPLC）. [Results]Over 200 strains of 20 endophytic fungi were isolated from the barks of Taxus mairei. A high taxol-yielding strain of taxol-producing fungus was obtained. Its Taxol content in culture medium reached 1. 003mg/L. [Conclusion] The observation of the morphology of individual and colonial features indicates that the isolated Taxol-yielding fungus is a new species of Alternaria.

关 键 词：真菌 分离和提纯 紫杉酚 类似物和衍生物 

分 类 号：R379[医药卫生—病原生物学]