硫酸铝对大蒜根尖细胞毒性效应的研究  

Cytogenetic Toxicity Effect of Aluminum Sulphate on Allium sativum L. Root Tip Cells

作　　者：秦永燕[1] 丹惠国[1] 刘瑞祥[1] 姚沁涛 

机构地区：[1]长治学院生物科学与技术系,山西长治046011 [2]长治一中生物组,山西长治046011

出　　处：《江西农业大学学报》2012年第6期1269-1272,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：山西省长治市科技计划项目(20123055);山西省高校科技研究开发基金资助项目(20081043);长治学院2009年校级科研课题(200913)

摘　　要：以不同浓度的硫酸铝为诱变剂,采用微核法和染色体畸变法,测定大蒜根尖细胞的微核率、有丝分裂指数和染色体畸变率。结果表明:在试验浓度范围内,随着处理浓度的升高,微核率呈先增加后减少;细胞有丝分裂指数呈先减少后增加,但均低于对照组,而且差异显著(P<0.05);硫酸铝可诱导大蒜根尖细胞染色体产生多种类型的畸变,如染色体粘连、染色体断片、染色体桥、染色体分离融合等现象,且畸变率随处理浓度的增加而升高。The cytogenetic toxicity effects of different concentrations of Al2（SO4）3 on Allium satvium L.root tip cells were studied.Micronucleus assay and chromosome aberration assay were used to determine the micronucleus rate,the mitotic index and chromosome aberration rate of A.satvium root tip cells.The result showed that：in the tested concentration range,the micronucleus rate increased first and then decreased with increasing concentration.The cell mitotic index decreased first and then increased,but lower than the control group（P0.05）.Various types of chromosome aberration were observed such as chromosome adhesion,chromosome fragments,chromosome bridges,chromosome separation fusion phenomenon and the distortion rate increased with increasing concentration.

关 键 词：硫酸铝 大蒜 微核率 有丝分裂指数 染色体畸变率 

分 类 号：Q944.6[生物学—植物学]