免疫多糖对日本蟳抗溶藻弧菌能力的影响  

Charybdis japonica: Influence of Immunopolysaccharide on Resistant to Vibrio alginolyticus

作　　者：石灏[1] 徐善良[1,2] 朱慧[1] 王瑞颖[1] 

机构地区：[1]宁波大学海洋学院 [2]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《河北渔业》2013年第1期1-5,共5页

基　　金：浙江省科技厅创新团队项目(2010R50029);宁波市创新团队项目(2011B81003)

摘　　要：通过对日本蟳(Charybdis japonica)分别注射生理盐水(对照组)、溶藻弧菌(攻毒组)、免疫多糖(免疫组)、免疫多糖+溶藻弧菌(免疫攻毒组)后,再测定4组肝胰腺及肌肉中溶菌酶(LZM)和超氧化物岐化酶(SOD)的活性变化,探讨免疫多糖对日本蟳抗溶藻弧菌能力的影响。结果表明:日本蟳肝胰腺中LZM和SOD的酶活均高于肌肉中的酶活,且免疫多糖对肝胰腺的免疫增强效应显著高于肌肉;免疫组肝胰腺和肌肉中LZM酶活,在注射后24h时显著升高至峰值(P<0.05),分别为(11.2±2.78)U/mg和(3.75±1.05)U/mg,肝胰腺和肌肉中的SOD酶活也在24h达到最高值,分别是对照组的2.2倍和1.4倍;免疫攻毒组在72h时,肝胰腺和肌肉中的LZM酶活分别高出攻毒组62.8%和75.2%,两组织中SOD酶活同样显著高于攻毒组(P<0.05),是攻毒组的3.5倍和4.2倍。由此可见,免疫多糖能提高日本蟳抗溶藻弧菌能力,且在72h达到最佳免疫保护效果。In order to explore influence of immunopolysaccharide on resistant to Vibrio alginolyticus of Charybdis japonica, C. japonicas were injected with saline（control group）, V. alginolyticus （bacterial infected group）, immunopolysaccharide （immune group）, immunopolysacchride and V. algino- lyticus （immune plus bacte：rial infected group）, and then the changes Of the lysozyme （LZM） and superoxide dismutase （SOD） in the hepatopancreas and muscle were assaied. The results showed as follows. the activities of LZM and SOD in hepatopancreas were higher than in the muscle, and the immune enhancement effect on the hepatopancreas was significantly higher than on the muscle. The LZM＇s activities of the immune group in the hepatopancreas and muscle were significantly elevated to the peak（P〈0.05） after C. japonica were injected at the 24h, which were （11.2 ±2.78）U/rag and （3.75±1.05） U/mg. And SODs activities of in the hepatopancreas and muscle also reached the peak at the 24h, which were 2.2 and 1.4 times as high as that of control group. At the 72h, LZM＇s activities in the hepatopancreas and muscle of the immune plus bacterial infected group were 62.8% and 75. 2% higher than those of the bacterial infected group. The SOD＇s activities were also significantly higher than those of bacterial infected group（P〈0.05）, which were 3.5 and 4.2 times as high as that of the bacterial infected group. Therefore, immunopolysaecharide makes a effective influence on resistant to Vibrio alginolyticus of C. japonica, and it can get the optimal immune protection at 72h.

关 键 词：日本蟳 免疫多糖 LZM SOD 溶藻弧菌 

分 类 号：S945[农业科学—水产养殖]