红景天苷对经紫外线辐射的成纤维细胞中Fas和Bcl-2mRNA表达的影响  

Effect of salidroside on Fas and Bcl-2 mRNA level in dermal fibroblasts under ultravidet radiation

作　　者：史飞[1] 蔡庆[1] 王毅侠[1] 刘雯[1] 蔡瑞康[1] 

机构地区：[1]空军总医院皮肤科,北京100142

出　　处：《中国医药》2013年第1期109-111,共3页China Medicine

摘　　要：目的观察红景天苷对经紫外线A／B（UVA／UVB）辐射的体外培养人皮肤成纤维细胞中Fas和Bcl-2mRNA表达的影响，探讨红景天苷抑制细胞凋亡与凋亡相关细胞因子的关系。方法在体外培养人皮肤成纤维细胞并随机分为正常对照组、红景天苷组、UVB组、UVB＋红景天苷组、UVA组、UVA＋红景天苷组6组，通过反转录聚合酶链反应法检测Fas和Bcl-2的mRNA表达。结果UVB、UVA辐射体外培养的成纤维细胞后，FasmRNA表达增加[（0．45±0．04），（0．44±0．04）比（0．13±0．03）]，Bcl-2mRNA表达减弱[0．17±O．01，0．17±0．03比（0．13±O．03）]；加入红景天苷后Fas和Bcl-2的mRNA表达分别降低[（0．17±0．04）比（0．45±0．04），0．18±0．03比0．44±0．04；（0．31±0．02）比（0．17±0．01），（0．28±0．04）比（0．17±O．03）]。结论红景天苷对紫外线引起的Fas和Bcl-2的mRNA表达具有抑制作用，延缓UVB、UVA辐射造成的细胞凋亡过程。Objective To explore the effect of salidroside on Fas and Bcl-2 mRNA level in fibroblast apoptosis that ultraviolet radiation B （UVB） and ultraviolet radiation A （UVA） irradiation can induce. Methods The fibroblast was divided into six groups randomly： control group, salidroside group, UVB group, UVA group, UVB group and UVA group treated with salidroside. The mRNA expression levels of Fas and Bcl-2 were determined. Results The expression of Fas and Bcl-2 mRNA Level were obviously increased and decreased after UVB and UVA irradiation compared with the control group [ 0.45± 0.04, 0.44± 0.04 vs 0.13 ± 0.03 ; 0.17± 0.01,0.17 ± 0.03 vs 0.31 ±0.031- After being treated with salidroside, the expression was decreased and increased [ 0.17 ± 0.04 vs 0.45±0.04, 0. 18±0.03 vs 0.44±0.04; 0.31 ±0.02 vs 0. 17 ±0. 18, 0.28±0.04 vs 0. 17±0.03]. Conclusions The UVB/UVA can induce fibroblast apoptosis by decreasing the mRNA expression of Fas （0.45 ± 0. 04/0.44 ± 0.04） and Bcl-2 （0.17 ±0.01/0.17 ± 0.03 ）. The salidroside can decrease the level of Fas and Bcl-2 mRNA and fibroblast apoptosis that UVB and UVA irradiation induce.

关 键 词：红景天苷 成纤维细胞 紫外线 

分 类 号：R285.5[医药卫生—中药学]