生物检测及分子诊断高端装备的研制及应用  

Research and application on high end equipment of biological detection and molecular diagnosis

作　　者：彭年才 李磊[1,2] 李政[1,2] 苗保刚 李明 赵垚[1,2] 蒋庄德 

机构地区：[1]西安交通大学机械工程学院,西安710049 [2]西安交通大学机械制造系统工程国家重点实验室,西安710049 [3]西安天隆科技有限公司,西安710049

出　　处：《中国工程科学》2013年第1期57-62,共6页Strategic Study of CAE

基　　金："863"国家高技术研究发展计划资助项目(2007AA042104;2012AA040504);国家科技支撑计划资助项目(2012BAI19B05);国家自然科学基金资助项目(61227018)

摘　　要：以生物检测与分子诊断技术发展为背景,总结了团队在医学诊断、疾控、食品安全、检疫检验、生物学医学科学研究等领域的分子诊断高端装备的研制情况,重点介绍了实时荧光定量聚合酶链式反应(PCR)分子诊断仪关键技术的研发及仪器工程化技术、检测性能的验证和在社会发展重点领域中的成功应用,手持式腺嘌呤核苷三磷酸(ATP)生物发光法细菌快速检测仪的研发与应用,以及后续的全自动核酸定量检测与高分辨分析设备研制。Based on biological detection and molecular diagnostics technology development, the author summarized high-end equipment independent research in medical diagnostics, disease control, food safety, quar- antine inspection, biology and medical research field of molecular diagnostics. The paper focused on research and development of key technology and instrumentation engineering technology, detection performance verification and successful application in the field of social development priorities of the real-time fluorescence quantitative polymerase chain reaction （PCR） nucleic acid detector, diagnosis of high-end equipment, the development and application and subsequent automatic nucleic acid directional monitoring with high-resolution analysis equipment development of handheld adenosine triphosphate （ATP） bioluminescence rapid detection of bacteria.

关 键 词：生物检测 分子诊断 实时荧光定量PCR ATP荧光检测 

分 类 号：Q78[生物学—分子生物学]