小单孢菌FIM10-2207生物转化卡那霉素B的初步研究  

The preliminary study on microbial conversion of kanamycin B by Micromonospora FIM10-2207

作　　者：杨良[1] 黄楷[2] 陈秀明[2] 陈晓明[2] 郑卫[1,2] 

机构地区：[1]福建医科大学药学院,福州350108 [2]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《中国抗生素杂志》2013年第1期22-25,共4页Chinese Journal of Antibiotics

基　　金：国家"十一五"重大专项支持(NO.2010ZX09401-403)

摘　　要：在卡那霉素B的微生物转化研究中,从台湾海峡海底沉积物中分离得到五株对卡那霉素B具有转化作用的菌株,选取其中转化率较高的一株菌,编号为FIM10-2207,经分类学研究鉴定为小单孢菌。初步考察了培养基的选择,底物卡那霉素B的初始浓度和添加时间这3个因素对转化率的影响,发现转化培养基C,添加时间32h和底物初始浓度1500μg/mL时,转化率较高。卡那霉素B经该菌株转化后,得到一个新化合物,波谱数据分析结果表明该产物为1,3″-二乙酰化卡那霉素B。In the research of microbial conversion of kanamycin B, five bacterial strains were isolated from a sediment soil of Taiwan strait. Among them, a higher conversion strain FIM10-2207 was identified as a Micromonospora after the taxonomic studies. After the initial studies on the effects of conversion yield by the choice of medium, initial concentration and addition time, the conversion yield was higher when addition time was 32h and the initial concentration of substrate was 1500μg/mL. A new chemical compound was obtained after the transformation on the kanamycin B and it was proved to be 1,3＂-diacetylated kanamycin B by spectra analyses.

关 键 词：卡那霉素B 微生物转化 小单孢菌 

分 类 号：TQ465[化学工程—制药化工]