放线菌FIM06-0063产生的安莎类化合物的分离、纯化及其结构鉴定  

Isolation, purification, and structure identification of ansamycin compounds from Actinomycetes FIM06-0063

作　　者：毛思雨[1] 陈宏[2] 陈丽[2,3] 王传喜[2,3] 陈晓明[2] 贾纬[2] 谢颖[2,3] 郑卫[1,2] 

机构地区：[1]福建医科大学药学院,福州350004 [2]福建省微生物研究所,福州350007 [3]福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《中国抗生素杂志》2013年第1期36-40,共5页Chinese Journal of Antibiotics

基　　金：国家"十一五"重大专项"微生物药物技术创新与新药创制产学研联盟"(2010ZX09401-403)的支持

摘　　要：目的分离、纯化、鉴定来自放线菌FIM06-0063发酵液中的安莎类化合物。方法采用大孔吸附柱层析、硅胶柱层析等方法对FIM06-0063菌株的次级代谢产物进行分离纯化,以紫外、质谱、核磁等方法对获得的组分进行结构鉴定。结果从FIM06-0063菌株发酵液中共分离到4个安莎类化合物,经波谱方法鉴定,分别为:9-甲氧基-安丝菌素P-3(9-methoxy-ansamitocinP-3,1),安丝菌素P-2(ansamitocin P-2,2),安丝菌素P-3(ansamitocin P-3,3)和安丝菌素P-4(ansamitocin P-4,4)。结论首次从微生物发酵液中分离得到化合物1,为一个新的天然产物。活性研究表明该化合物对HL60肿瘤细胞具有增殖抑制活性,其IC50值为72.68nmol/L。Objective To isolate, purify and identify the structure of ansamycin compounds from the fermentation broth ofActinomycetes FIM06-0063. Methods The secondary metabolites were isolated and purified by macroporous resin column chromatography, silica gel chromatography and other ways. The chemical structures were identified by UV, MS and NMR spectra. Results Four compounds isolated from the fermentation broth of FIM06- 0063 were identified as 9-methoxy-ansamitocin P-3 （1）, ansamitocin P-2 （2）, ansamitocin P-3 （3） and ansamitocin P-4 （4） on the basis of spectral data. Conclusion Compound 1 is a new natural product isolated from the broth of strain FIM06-0063 and showes growth inhibitory activity against the tumor cell lines HL60 with IC50 values of 72.68nmol/L.

关 键 词：放线菌 安丝菌素 分离纯化 结构鉴定 

分 类 号：Q501[生物学—生物化学]