双亲性氧化石墨烯的合成及生物相容性  

Synthesis and Biocompatibility of Amphiphilic Graphene Oxide

作　　者：李宁[1,2,3] 张琦[2] 张庆庆[2,3] 宋琴[2] 齐琳[2] 王龙[2,3] 唐明亮[2] 靳刚[1,3] 程国胜[2,3] 

机构地区：[1]中国科学院力学研究所,北京100190 [2]中国科学院苏州纳米技术与纳米仿生研究所纳米生物医学部,苏州215123 [3]中国科学院大学,北京100049

出　　处：《高等学校化学学报》2013年第1期50-54,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金青年科学基金(批准号:31101059)资助

摘　　要：以六亚甲基二异氰酸酯(HMDI)为偶联剂,通过与氧化石墨烯(GO)中的羧基或羟基反应,形成酰胺键或氨基甲酸酯键活化GO,然后与TWEEN 80中的羟基反应,将双亲性TWEEN分子偶联于GO表面,制备了双亲性GO.所制备的TWEEN 80接枝GO在水、氯仿和己烷等溶剂中均可稳定分散.以PC12及HeLa细胞为细胞模型,考察TWEEN 80接枝GO所制薄膜的细胞毒性,形态学观察及细胞活性检测(Calcein AM&EthD-1荧光染色及MTT检测)结果表明其具有良好的生物相容性.Amphiphilic functionalization of graphene oxide（GO） is of the essence for its biomedical applica- tions. TWEEN 80-grafted GO was synthesized by a two-step approach. In step 1, GO surface was activated with hexamethylene diisocyanate （HMDI） through a reaction between GO carboxyl or hydroxyl groups and iso- cyanate. HMDI-activated GO was then grafted by TWEEN 80 in step 2, resulting in amphiphilic GO nano- sheets. The fabricated TWEEN 80-grafted GO exhibited excellent dispersibility in various solvents, such as water, chloroform and n-hexane. Furthermore, PC12 and HeLa cells were cultured on TWEEN 80-grafted GO film to assess its cytotoxicity. Cell morphology analysis and cell viability assay（ Calcein AM ＆EthD-1 staining and MTT assay） indicated that the as-synthesized amphiphilic GO had good biocompatibility.

关 键 词：双亲性氧化石墨烯 生物相容性 TWEEN 80 六亚甲基二异氰酸酯 

分 类 号：O613.71[理学—无机化学]