非标记型血小板衍生生长因子核酸适体传感器  

Preparation of Label-free Aptasensor for the Detection of Platelet-derived Growth Factor

作　　者：赵春玲[1] 华梅[1] 赵晓慧[1] 黄亮亮[1] 冯亚娟[1] 牛司朋[1] 符雪文[1] 袁聪[1] 杨云慧[1] 

机构地区：[1]云南师范大学化学化工学院,昆明650500

出　　处：《高等学校化学学报》2013年第1期61-66,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20865006;21165023)资助

摘　　要：合成了介孔二氧化硅负载金纳米颗粒(Au-MSN),通过壳聚糖(CHIT)将Au-MSN固定到裸玻碳电极表面,采用自组装法将带巯基的血小板衍生生长因子(PDGF)核酸适体固定到Au-MSN修饰过的玻碳电极表面,制得PDGF核酸适体传感器.以亚甲基蓝作为电化学活性嵌入剂,通过检测核酸适体与目标分析物PDGF特异性结合前后亚甲基蓝电信号的变化,实现了对PDGF的定量检测.考察了缓冲溶液的pH、扫描速度及PDGF培育时间等条件对检测结果的影响.结果表明,在pH为7.6时,该传感器的检测范围为0.1pg/mL～1μg/mL,检出限为0.03 pg/mL.该传感器制作简单、成本低廉、灵敏度高且稳定性好.Gold nanoparticles immobilized upon mesoporous silica （Au-MSN）were synthesized. In the pre- sence of chitosan（CHIT） , Au-MSN were immobilized on the surface of glassy carbon electrode. Then, the thiolated platelet-derived growth factor （PDGF） aptamer was immobilized on the electrode surface via self- assembled technology. Using methylene blue as electroactive intercalator, the quantitative determination of PDGF was carried out based on the increase of electrical signal of methylene blue after the combination of aptamer with target PDGF. The effects of the pH of buffer, incubation time etc. on the signal of the electrode were investigated. The results showed that the biosensor exhibits excellent linear response to PDGF in the range of 0. 1 pg/mL--1 μg/mL with a detection limit of 0.03 pg/mL. This electrochemical aptasensor showed simplicity, low cost, high sensitivity and stability.

关 键 词：电化学 介孔二氧化硅 核酸适体 血小板衍生生长因子(PDGF) 亚甲基蓝 

分 类 号：O657.1[理学—分析化学]