可降解聚合物包覆次血红素六肽微球的制备及生物活性  

Preparation and Bioactivities of DhHP-6-loaded Biodegradable Polymeric Microspheres

作　　者：赵天倚[1] 赵继鹏[1] 雷莉妍[1] 陈晓光[1] 王立成[2] 陈学思[3] 王丽萍[1] 

机构地区：[1]吉林大学生命科学学院 [2]吉林大学化学学院,长春130012 [3]中国科学院长春应用化学研究所,高分子物理和化学国家重点实验室,长春130022

出　　处：《高等学校化学学报》2013年第1期236-241,共6页Chemical Journal of Chinese Universities

基　　金：吉林省自然科学基金(批准号:201015171);吉林省医药产业发展专项(批准号:YYZX201150-2);吉林省科技发展计划项目(批准号:201205017)资助

摘　　要：采用水包油包水(W1/O/W2)复乳溶剂挥发法制备了包覆次血红素六肽(DhHP-6)的2种聚酯微球.通过扫描电子显微镜、体外缓释行为、高效液相(HPLC)检测、酶活力测定和初步的动物实验等表征,综合评价了载药微球的体外释放及体内生物活性.实验结果表明,载药微球球体圆整,粒径分布均匀,载药量高,能够实现体外缓释,并对细胞过氧化损伤和小鼠脑缺血损伤均有一定的保护作用.Two polyester microspheres were prepared by a water-in-oil-in-water（ W1/O/W2 ） double emulsion solvent evaporation and deuterohaemin-β-AHTVEK（DhHP-6） was encapsulated to the polyester microspheres by a technique with good sphericity and high efficiency. The in vitro release profiles and in vivo biological activity of the polyester microspheres were evaluated by scanning electron microscope （SEM）, in vitro release, high performance liquid（ HPLC）, enzyme activity detection and animal experiments. The data suggested that the drug-loaded polyester microspheres were spherical morphology with narrow size distribution, and had high entrapment capacity with great in vitro release profiles. Furthermore, the drug-loaded polyester microspheres had protective effects on cell injury induced by hydrogen peroxide and cerebral ischemic injury in mice.

关 键 词：载药微球 次血红素六肽 聚乙交酯-丙交酯 聚丙交酯-乙交酯 聚乙二醇 缓释 

分 类 号：O633.221[理学—高分子化学]