基于数据挖掘和网络分析的“脑心同治”分子机制研究  

Molecular mechanism research on simultaneous therapy of brain and heart based on data mining and network analysis

作　　者：陈迪[1] 卢朋[1,2] 张方博[2] 唐仕欢[2] 杨洪军[1] 

机构地区：[1]中国科学院自动化研究所,北京100190 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2013年第1期91-98,共8页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2011ZX09201-201-26);国家自然科学基金项目(81203005);陕西省科技统筹创新工程计划项目(2011KTZB03-01-04)

摘　　要：研究从分子层面出发,结合利用信息技术中的网络分析、聚类和关联规则分析方法,找到步长脑心通这一实现脑心同治的方剂中的化合物成分共同作用于人体内的主要靶标组合,并对比分析这些主要成分在基于冠心病和中风病相关的蛋白质交互作用网络中作用的节点的异同。结果从分子层面上找出该方的主要成分对于2种疾病频繁作用的共同靶标及对于2种疾病分别作用到的不同靶标,同时确定了主要成分在作用于2种疾病共同靶标的同时也间接影响到不同靶标,从而解释其脑心同治的分子作用机制。Objective： The theory of treating heart and brain simultaneously is from the theory of traditional Chinese medicine, and there aren＇t enough explanations for this theory from the perspective of molecular mechanism. As one successful case of this theory, the Chinese medicine formula Buchang Naoxintong can achieve the goal of treating coronary heart disease and stroke at the same time. To illustrate the mechanism of the theory of treating heart and brain simultaneously, it is necessary to find out the molecular mechanism of this formula. Method： Using the network analysis method, together with two data mining methods-clustering and apriori algorithm, the frequent gene combinations interfered by the chemicals of the formula based on the protein-protein interaction networks relat- ed with coronary heart disease and stroke disease were figured out respectively. To find out the molecular mechanism of the theory of treating heart and brain simultaneously, the results got from two diseases were compared and analyzed. Result： Based on comparing two results from these two different diseases, the mechanism of the theory of treating heart and brain simultaneously was explained from molecular level by finding out key genes targeted by the components of this formula for both diseases and some particular genes interfered by the components for each disease. In addition, genes interfered indirectly by the chemicals for different diseases were found out based on the protein-protein inter- action network. Conclusion： It can help to explain the molecular mechanism of the theory by our methods. By finding out the molecular mechanism of this theory, it can promote the progress of combination of Chinese traditional and Western medicine.

关 键 词：网络分析 数据挖掘 脑心同治 步长脑心通方 分子作用机制 

分 类 号：R285[医药卫生—中药学]